Differential effects of GDNF and BDNF on cultured ventral mesencephalic neurons

Previous studies have shown that brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) can enhance the survival of dopaminergic neurons in the ventral mesencephalon (VM). Here we compared several non-survival functions of the two factors in VM neurons in cul...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 1999-03, Vol.66 (1), p.62-70
Main Authors: Feng, Linyin, Wang, Chang-Yu, Jiang, Hao, Oho, Chikara, Mizuno, Keiko, Dugich-Djordjevic, Millicent, Lu, Bai
Format: Article
Language:English
Subjects:
Actins - biosynthesis
Animals
BDNF
Biological and medical sciences
Brain-Derived Neurotrophic Factor - pharmacology
Calbindins
Cell Differentiation - drug effects
Cell physiology
Cell Survival - drug effects
Cells, Cultured
Ciliary Neurotrophic Factor
Dopamine - metabolism
Fasciculation
Fetus - cytology
Fundamental and applied biological sciences. Psychology
gamma-Aminobutyric Acid - metabolism
GDNF
Glial Cell Line-Derived Neurotrophic Factor
Membrane Proteins - biosynthesis
Mesencephalic
Mesencephalon - cytology
Molecular and cellular biology
Nerve Growth Factors
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - pharmacology
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
Neuroprotective Agents - pharmacology
R-SNARE Proteins
Rats
Rats, Sprague-Dawley
Responses to growth factors, tumor promotors, other factors
S100 Calcium Binding Protein G - biosynthesis
Synapses - metabolism
Synapsins - biosynthesis
Synaptic protein
Synaptosomal-Associated Protein 25
Transmitter release
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Summary:Previous studies have shown that brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) can enhance the survival of dopaminergic neurons in the ventral mesencephalon (VM). Here we compared several non-survival functions of the two factors in VM neurons in culture. We found that both BDNF and GDNF elicited an increase in the depolarization-induced release of dopamine, but had no effect on GABA release, in the VM cultures. BDNF, but not GDNF, significantly enhanced the expression of the calcium binding protein calbindin and synaptic protein SNAP25. In contrast, treatment of the cultures with GDNF, but not BDNF, elicited a marked fasciculation of the processes of the VM neurons. Thus, although both act on VM neurons, BDNF and GDNF have distinct functions.
ISSN:0169-328X
1872-6941
DOI:10.1016/S0169-328X(99)00015-7