Detection of chromium allergy by cellular in vitro methods

Summary Background The standard assay for the detection of chromium sensitization, the patch test, does not allow discrimination between patients with and without clinical symptoms of allergy. Objective The aim of this study was to prove whether cellular in vitro tests are predictive of chromium all...

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Bibliographic Details
Published in:Clinical and experimental allergy 2008-09, Vol.38 (9), p.1468-1475
Main Authors: Lindemann, M., Rietschel, F., Zabel, M., Grosse-Wilde, H.
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Cells, Cultured
Chlorides - immunology
chromium allergy
Chromium Compounds - immunology
Cytokines - immunology
Dermatitis, Allergic Contact - diagnosis
Dermatitis, Allergic Contact - immunology
ELISpot
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
in vitro cytokine production
in vitro lymphocyte proliferation
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - immunology
Lymphocyte Activation
Male
Middle Aged
patch test
Patch Tests
Potassium Dichromate - immunology
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Summary:Summary Background The standard assay for the detection of chromium sensitization, the patch test, does not allow discrimination between patients with and without clinical symptoms of allergy. Objective The aim of this study was to prove whether cellular in vitro tests are predictive of chromium allergy. Methods Chromium‐sensitized volunteers with and without clinically manifest allergy and non‐sensitized healthy controls (n=37, 19, and 26, respectively) were analysed by cellular in vitro methods using tri‐ and hexavalent chromium (chromium chloride and potassium dichromate) as stimuli. The results were correlated with clinical and anamnestic data. Results Sensitized individuals with an allergy displayed significantly higher lymphocyte transformation test (LTT) responses than sensitized volunteers without allergy and controls (P
ISSN:0954-7894
1365-2222
DOI:10.1111/j.1365-2222.2008.02970.x