Molecular and functional study of AQY1 from Saccharomyces cerevisiae: role of the C-terminal domain

The yeast YPR192w gene, which encodes a protein (Aqy1p) with strong homology to aquaporins (AQPs), was cloned from nine S. cerevisiae strains. The osmotic water permeability coefficient (Pf) of X. laevis oocytes expressing the gene cloned from the Sigma1278b strain (AQY1-1) was 5.7 times higher than...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-04, Vol.257 (1), p.139-144
Main Authors: Laize, V. (Goteborg University, Sweden.), Gobin, R, Rousselet, G, Badier, C, Hohmann, S, Ripoche, P, Tacnet, F
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
AQUAPORINS
Aquaporins - chemistry
Aquaporins - genetics
Aquaporins - metabolism
Blotting, Western
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Cell Membrane Permeability - drug effects
Cloning, Molecular
Fungal Proteins - chemistry
Fungal Proteins - metabolism
Glycerol - metabolism
Mercuric Chloride - pharmacology
Molecular Sequence Data
Oocytes - cytology
Oocytes - metabolism
Oocytes - ultrastructure
Osmolar Concentration
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Sequence Deletion
Sorbitol - metabolism
Spheroplasts - drug effects
Spheroplasts - metabolism
Temperature
Urea - metabolism
Water - metabolism
Xenopus
Xenopus laevis
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Description
Summary:The yeast YPR192w gene, which encodes a protein (Aqy1p) with strong homology to aquaporins (AQPs), was cloned from nine S. cerevisiae strains. The osmotic water permeability coefficient (Pf) of X. laevis oocytes expressing the gene cloned from the Sigma1278b strain (AQY1-1) was 5.7 times higher than the Pf of oocytes expressing the gene cloned from other strains (AQY1-2). Aqy1-1p, initially cloned without its C-terminus (Aqy1-1DeltaCp), mediated an approximately 3 times higher water permeability than the full-length protein. This corresponds to a 3-fold higher protein density in the oocyte plasma membrane, as shown by freeze-fracture electron microscopy. Pf measurements in yeast spheroplasts confirmed the presence of functional water channels in Sigma1278b and a pharmacological study indicated that this strain contains at least a second functional aquaporin.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0425