In vivo operation of the pentose phosphate pathway in frog oocytes is limited by NADP + availability

Evolution of CO 2 from labelled glucose microinjected into frog oocytes in vivo may be ascribed to the pentose-P pathway, as measured by radioactive CO 2 production from [1- 14C] and [6- 14C]glucose. Coinjection of NADP + and [ 14C]glucose significantly stimulated 14CO 2 production. The effect depen...

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Bibliographic Details
Published in:FEBS letters 1999-03, Vol.446 (1), p.149-152
Main Authors: Preller, Ana, Guixé, Victoria, Ureta, Tito
Format: Article
Language:English
Subjects:
Animals
Female
Frog oocyte
Glucose - metabolism
NADP
NADP - metabolism
Oocytes - metabolism
Pentose Phosphate Pathway
Rana pipiens
Substrate Specificity
Xenopus laevis
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Summary:Evolution of CO 2 from labelled glucose microinjected into frog oocytes in vivo may be ascribed to the pentose-P pathway, as measured by radioactive CO 2 production from [1- 14C] and [6- 14C]glucose. Coinjection of NADP + and [ 14C]glucose significantly stimulated 14CO 2 production. The effect depends on the amount of NADP + injected, half maximal stimulation being obtained at 0.13 mM. The increase in CO 2 production was also observed with microinjected glucose-1-P, glucose-6-P or fructose-6-P used as substrates. Phenazine methosulfate, mimicked the effects of NADP +. A high NADPH/NADP + ratio of 4.3 was found in the cells, the intracellular concentration of NADP + being 19 μM.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)00192-1