EXAMINATION OF ANTIBACTERIAL ACTIVITY OF THE PHOTOACTIVATED ARGININE HAEMATOPORPHYRIN DERIVATIVE

• Published in: Pharmacological research 1999-03, Vol.39 (3), p.181-184
• Main Authors: LASOCKI, KRZYSZTOF, SZPAKOWSKA, MA, GRZYBOWSKI, JACEK, GRACZYK, ALFREDA
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Arginine - chemistry; Colony-Forming Units Assay; haematoporphyrin derivative, antibacterial photodynamic effect,S. aureus,P. aeruginosa; Hematoporphyrins - pharmacology; Light; Mice; Muscles - drug effects; Muscles - microbiology; Photochemistry; Pseudomonas aeruginosa - drug effects; Staphylococcus aureus - drug effects
• ISSN: 1043-6618; 1096-1186
• DOI: 10.1006/phrs.1998.0431

The antibacterial photodynamic effect of arginine haematoporphyrin derivative (HpD-Arg2) was investigated using two methods. The effect proved to be highly antibacterial when it was employed againstP. aeruginosaandS. aureuscells floating in the nutrient broth. The reduction of approximately four logarithms of CFU ml−1after light exposition, as compared to a dark control, was observed. In the second step of the study the photodynamic effect was employed against the same strains of bacteria after their colonization on isolated mouse muscles. In this case the antibacterial effect was observed as well. However, the reduction of CFU ml−1achieved was much lower and reached one logarithm only. The optimal concentration of photoactivated HpD-Arg2forS. aureusandP. aeruginosawas 25 μg ml−1and 800 μg ml−1for the floating bacteria, and 200 μg ml−1and 1000 μg ml−1for sessile bacteria, respectively. We have concluded from our study that the antibacterial effect investigated may offer, in the future, an alternative method for treatment of the tissues superficially infected with pathogens resistant to antibiotics. However, further studies in this field are necessary.