Prediction and diagnosis of attachment loss by enhanced chemiluminescent assay of crevicular fluid alkaline phosphatase levels

. The current study aimed to apply a novel enhanced chemiluminescence assay in the analysis of gingival crevicular fluid (GCF) alkaline phosphatase (ALP) levels from patients with untreated adult periodontitis. 3666 sites in 25 patients were monitored prior to and after attachment loss was detected...

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Bibliographic Details
Published in:Journal of clinical periodontology 1999-03, Vol.26 (3), p.190-198
Main Authors: Chapple, I. L. C., Garner, I., Saxby, M. S., Moscrop, H., Matthews, J. B.
Format: Article
Language:English
Subjects:
Adult
alkaline phosphatase
Alkaline Phosphatase - analysis
attachment loss
Chronic Disease
chronic periodontitis
Dentistry
Disease Progression
enhanced chemiluminescence
Follow-Up Studies
Forecasting
gingival crevicular fluid
Gingival Crevicular Fluid - enzymology
Gingival Hemorrhage - diagnosis
Gingival Hemorrhage - enzymology
Humans
Longitudinal Studies
Luminescent Measurements
Pain Measurement
Periodontal Attachment Loss - diagnosis
Periodontal Attachment Loss - enzymology
Periodontal Pocket - diagnosis
Periodontal Pocket - enzymology
Periodontics - instrumentation
Periodontitis - enzymology
Periodontitis - therapy
Sensitivity and Specificity
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Summary:. The current study aimed to apply a novel enhanced chemiluminescence assay in the analysis of gingival crevicular fluid (GCF) alkaline phosphatase (ALP) levels from patients with untreated adult periodontitis. 3666 sites in 25 patients were monitored prior to and after attachment loss was detected with a Florida disc probe. Parameters assessed were, relative attachment level, probing pocket depth, occurrence of bleeding on probing (single episode), GCF volume (μl), total ALP levels (μIU/30 s sample time) and ALP concentration (IU/l). After recruiting patients to the study, all measures were taken at baseline and 3 months later, prior to the institution of non‐surgical periodontal therapy at active sites. Thresholds for determining attachment loss were calculated using a modification of the tolerance method. The mesio‐buccal sites of all teeth had GCF samples collected. The size of individual patient thresholds used to define whether attachment loss had occurred, was dependent upon the discomfort felt by that patient during electronic probing, with a positive correlation existing between discomfort on probing (10 cm visual analogue scale) and threshold size (R=0.52, p
ISSN:0303-6979
1600-051X
DOI:10.1034/j.1600-051X.1999.260310.x