Radiation-Induced Recombination Is Dependent on Ku80

We have recently shown that irradiating cells prior to transfection induces recombination, as manifested by increased stable transduction of both plasmid and adenoviral vectors. We hypothesized that Ku proteins, which have previously been shown to be involved in both recombination and the repair of...

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Bibliographic Details
Published in:Radiation research 1999-04, Vol.151 (4), p.408-413
Main Authors: Stevens, Craig W., Stamato, Thomas D., Mauldin, Stanley K., Getts, Robert C., Zeng, Ming, Cerniglia, George J.
Format: Article
Language:English
Subjects:
Animals
Antigens, Nuclear
Biological and medical sciences
Cell lines
Cell nucleus
CHO Cells
Cricetinae
DNA
DNA Damage
DNA Helicases
DNA-Binding Proteins - analysis
DNA-Binding Proteins - physiology
Fundamental and applied biological sciences. Psychology
Humans
Ionizing radiation
Irradiation
Ku Autoantigen
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Nuclear Proteins - analysis
Nuclear Proteins - physiology
Plasmids
Proteins
Radiation Tolerance
Recombination, Genetic - radiation effects
Space life sciences
Transfection
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Summary:We have recently shown that irradiating cells prior to transfection induces recombination, as manifested by increased stable transduction of both plasmid and adenoviral vectors. We hypothesized that Ku proteins, which have previously been shown to be involved in both recombination and the repair of DNA damage after irradiation, would likely be important mediators of radiation-induced recombination. The present work demonstrates that Ku80 is essential for radiation-induced recombination. While human and hamster Ku80 are equally effective at restoring the transfection efficiency and radiation resistance of xrs-5 cells, human Ku80 is much more effective at radiation-induced recombination than hamster Ku80. This difference is not due to differences in Ku80 expression or DNA end-binding activity, but it may be due to structural differences between human and hamster Ku80.
ISSN:0033-7587
1938-5404
DOI:10.2307/3579827