Katacalcin and calcitonin immunoreactivity in different types of leukocytes indicate intracellular procalcitonin content

Purpose: Procalcitonin is a new marker of severity of nonviral, in particular, bacterial infections. In respect of sepsis its site of production remains unknown. This study was carried out to determine whether subsets of human leukocytes contain procalcitonin. Materials and Methods: Blood samples of...

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Bibliographic Details
Published in:Journal of critical care 1999-03, Vol.14 (1), p.29-33
Main Authors: Oberhoffer, Mathias, Vogelsang, Heinz, Jäger, Lothar, Reinhart, Konrad
Format: Article
Language:English
Subjects:
Antibody Formation
Bacterial Infections - diagnosis
Biomarkers
Calcitonin - blood
Calcitonin - immunology
Calcitonin Gene-Related Peptide
Humans
Immunohistochemistry
Leukocytes - metabolism
Peptide Fragments - blood
Peptide Fragments - immunology
Predictive Value of Tests
Protein Precursors - blood
Severity of Illness Index
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Summary:Purpose: Procalcitonin is a new marker of severity of nonviral, in particular, bacterial infections. In respect of sepsis its site of production remains unknown. This study was carried out to determine whether subsets of human leukocytes contain procalcitonin. Materials and Methods: Blood samples of 17 patients who had demonstrated various degrees of serum pro-calcitonin levels on the day before evaluation were analyzed for serum procalcitonin by immunoluminometry and for intracellular reaction of monocytes, granulocytes, B-, and T-lymphocytes against katacalcin- or calcitonin-sensitive antibodies. Katacalcin and calcitonin are part of the procalcitonin molecule. Associations of these reactions with serum procalcitonin levels as well as differences between groups with a normal or elevated serum level were analyzed. Results: Intracellular antibody reaction against katacalcin was demonstrated in all cell types. We also found a lower rate of intracellular antibody reaction against calcitonin. Associations of serum procalcitonin with the two antibody reactions were demonstrated. Differences in intracellular reactions in the group with elevated serum procalcitonin were seen with both antibodies compared with a normal control. Conclusion: Intracellular antibody reaction against katacalcin supports the notion that various types of leukocytes contain procalcitonin.
ISSN:0883-9441
1557-8615
DOI:10.1016/S0883-9441(99)90005-9