Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2

• Published in: Veterinary microbiology 1999-03, Vol.65 (3), p.233-240
• Main Authors: Zamri-Saad, M., Effendy, A.W.M., Israf, D.A., Azmi, M.L.
• Format: Article
• Language: English
• Subjects: Administration, Intranasal; Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies, Bacterial - biosynthesis; Bacterial diseases; Biological and medical sciences; Bronchoalveolar Lavage - veterinary; CAPRIN; CAPRINOS; Enzyme-Linked Immunosorbent Assay - veterinary; Experimental bacterial diseases and models; Formaldehyde - chemistry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Goat; Goat Diseases - immunology; Goat Diseases - microbiology; GOATS; IMMUNE RESPONSE; Immune response-goat; Immunity, Cellular - immunology; Immunobiology; IMMUNOGLOBULINE; IMMUNOGLOBULINS; Immunoglobulins - analysis; Immunoglobulins - blood; Infectious diseases; INMUNOGLOBULINA; Intranasal stimulation; Lymphoid Tissue - immunology; Lymphoid Tissue - microbiology; Mannheimia haemolytica - immunology; Medical sciences; Organs and cells involved in the immune response; PASTEURELLA HAEMOLYTICA; Pasteurella Infections - immunology; Pasteurella Infections - veterinary; REPONSE IMMUNITAIRE; RESPIRATORY SYSTEM; Respiratory System - immunology; Respiratory System - microbiology; Respiratory Tract Diseases - immunology; Respiratory Tract Diseases - veterinary; RESPUESTA INMUNOLOGICA; SISTEMA RESPIRATORIO; SYSTEME RESPIRATOIRE
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/S0378-1135(98)00298-3

A study to determine the immunoglobulin and cellular responses in the respiratory tract of goats following intranasal exposures to formalin-killed Pasteurella haemolytica A2 was carried out. Forty-two goats were divided into two groups. Goats in Group 1 were subjected to double intranasal exposures to formalin-killed P. haemolytica A2 while goats in Group 2 were the unexposed control. Prior to and at weekly intervals post-exposure, three goats from each group were killed, serum samples were collected while the lungs were flushed with 50 ml normal saline before the right apical lobes were fixed in 10% buffered formalin. Both serum and lung lavage fluid were subjected to enzyme-linked immunosorbent assay (ELISA) to determine the levels of IgA, IgM and IgG while the formalin-fixed tissues were examined histologically. IgA levels in the lung lavage fluid increased rapidly to reach a significantly ( p < 0.05) high level as early as Week 2 post-exposure and remained significantly ( p < 0.05) high throughout the study period. The IgM levels increased at an intermediate rate to reach a significantly ( p < 0.05) high level at Week 3 post-exposure before they decreased to an insignificant ( p > 0.05) level the following week and the weeks thereafter. IgG levels increased gradually and only reached a significantly ( p < 0.01) high level at Weeks 5 and 6 of the study. The size of the bronchus-associated lymphoid tissue (BALT) and the number of lymphocytes in BALT increased significantly from Week 2 and remained high thereafter. However, differences in the numbers of BALT were insignificant ( p > 0.05) initially before becoming significantly ( p < 0.05) high at Weeks 5 and 6. The BALT responses were parallel to those of immunoglobulins in the lung lavage fluid.