Role of intracellular calcium in Pasteurella haemolytica leukotoxin-induced bovine neutrophil leukotriene B4 production and plasma membrane damage

Isolated neutrophils were used to study the intracellular calcium ([Ca2+]i) dependency of Pasteurella haemolytica leukotoxin-induced production of leukotriene B4 and plasma membrane damage. Exposure of neutrophils to leukotoxin caused a rapid and concentration-dependent increase in [Ca2+]i, followed...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-03, Vol.172 (2), p.123-129
Main Authors: Cudd, L, Clarke, C, Clinkenbeard, K, Shelton, M, Clinkenbeard, P, Murphy, G
Format: Article
Language:English
Subjects:
animal diseases
animal health
Animals
bacterial infections
Bacterial Toxins - pharmacology
Bacteriology
Biological and medical sciences
Calcium (extracellular)
Calcium (intracellular)
Calcium - physiology
Calcium channels
Calcium channels (voltage-gated)
Calcium influx
Calcium ions
Cations
Cattle
Cell Membrane - drug effects
Damage
Dependence
Divalent cations
Exotoxins - pharmacology
Fundamental and applied biological sciences. Psychology
Intracellular
Intracellular calcium
Leukocytes (neutrophilic)
Leukotoxin
Leukotriene B4
Leukotriene B4 - metabolism
Luminescent Measurements
Mannheimia haemolytica - metabolism
Membranes
Microbiology
Neutrophils
Neutrophils - drug effects
Neutrophils - metabolism
Pasteurella haemolytica
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Time Factors
Verapamil
Verapamil - pharmacology
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Summary:Isolated neutrophils were used to study the intracellular calcium ([Ca2+]i) dependency of Pasteurella haemolytica leukotoxin-induced production of leukotriene B4 and plasma membrane damage. Exposure of neutrophils to leukotoxin caused a rapid and concentration-dependent increase in [Ca2+]i, followed by simultaneous plasma membrane damage and production of leukotriene B4. Removal of extracellular Ca2+, replacement of Ca2+ with other divalent cations, or exposure to high concentration of verapamil, an inhibitor of voltage-dependent calcium channels, inhibited leukotoxin-induced increases in [Ca2+]i, leukotriene B4 production, and membrane damage, thus indicating that influx of extracellular Ca2+ is necessary to produce these leukotoxin-induced neutrophil responses.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13459.x