Prolonged release and c‐kit expression of haemopoietic precursor cells mobilized by stem cell factor and granulocyte colony stimulating factor

Mobilization of haemopoietic precursor cells into the circulation by the combination of cytokines, stem cell factor (SCF) and G‐CSF in previously untreated patients with carcinoma of the breast resulted in increased yield of collected peripheral blood precursor cells (PBPC). This mobilization of PBP...

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Bibliographic Details
Published in:British journal of haematology 1999-03, Vol.104 (4), p.778-784
Main Authors: Roberts, M. M., Swart, B. W., Simmons, P. J., Basser, R. L., Begley, C. G., To, L. B.
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antigens, CD34
Biological and medical sciences
Bone marrow, stem cells transplantation. Graft versus host reaction
Breast Neoplasms - metabolism
Breast Neoplasms - therapy
Cohort Studies
c‐kit expression
Drug Combinations
Female
Flow Cytometry
Granulocyte Colony-Stimulating Factor - therapeutic use
Hematopoietic Stem Cell Mobilization - methods
Hematopoietic Stem Cells - metabolism
Humans
Immunophenotyping
Leukapheresis - methods
Medical sciences
mobilization
Phenotype
Proto-Oncogene Proteins c-kit - metabolism
stem cell factor
Stem Cell Factor - therapeutic use
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:Mobilization of haemopoietic precursor cells into the circulation by the combination of cytokines, stem cell factor (SCF) and G‐CSF in previously untreated patients with carcinoma of the breast resulted in increased yield of collected peripheral blood precursor cells (PBPC). This mobilization of PBPC by SCF with G‐CSF lasted several days after ceasing the cytokines in comparison to the rapid fall of PBPC after ceasing G‐CSF. Possible mechanisms for this increased and prolonged mobilization were investigated. Immunological phenotyping with CD38, Thy‐1 and MDR‐1 of the CD34‐positive mobilized PBPC detected no difference in maturity compared to PBPC mobilized by G‐CSF alone. However, the down‐regulation of c‐kit, which is associated with the mechanism of mobilization, was much greater in the PBPC mobilized by SCF and G‐CSF. The potential clinical implication of increased and prolonged mobilization is increased yield, allowing transplantation of heavily pretreated patients, transplantation with PBPC from a single apheresis, or PBSC support for multiple courses of high‐dose therapy from one mobilization procedure.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1999.01231.x