Antibody‐mediated inhibition of the human alloimmune response to platelet transfusion in Hu‐PBL‐SCID mice

Severe combined immune deficient (SCID) mice were engrafted with human (Hu) peripheral blood lymphocytes (PBL) from a previously alloimmunized donor and transfused with HLA‐mismatched platelets. We have previously shown this to be a useful model for platelet transfusion. These engrafted mice (Hu‐PBL...

Full description

Saved in:
Bibliographic Details
Published in:British journal of haematology 1999-03, Vol.104 (4), p.919-924
Main Authors: Crow, Andrew R., Freedman, John, Hannach, Barbara, Blanchette, Victor, Lazarus, Alan H.
Format: Article
Language:English
Subjects:
alloantibodies
alloimmunization
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Antibody Formation
Autoimmunity - immunology
B-Lymphocytes - cytology
Biological and medical sciences
Blood Platelets - immunology
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Cell Division
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Hematology
HLA
Humans
Immunoglobulin G - analysis
Isoantibodies - analysis
Isoantibodies - immunology
Medical sciences
Mice
Mice, SCID
Platelet Transfusion
platelets
SCID
T-Lymphocytes - cytology
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Severe combined immune deficient (SCID) mice were engrafted with human (Hu) peripheral blood lymphocytes (PBL) from a previously alloimmunized donor and transfused with HLA‐mismatched platelets. We have previously shown this to be a useful model for platelet transfusion. These engrafted mice (Hu‐PBL‐SCID mice) produced high levels of alloantibody in response to standard platelet preparations. However, when the first platelet challenge was presensitized with anti‐HLA antibody and then transfused there was a marked reduction in the amount of alloantibody produced to five subsequent untreated platelet transfusions. Platelets pretreated with platelet‐specific anti‐HPA‐1a (PLA1) sera did not induce a decrease in the anti‐HLA alloantibody response. This demonstrated that platelet‐induced HLA alloimmunization can be blocked by anti‐HLA antibody‐sensitized cells.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1999.01237.x