Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas

Summary Objectives  The pancreatic ducts, endocrine islets and exocrine acini are three functionally related components. From birth to adulthood, the islets and ducts are regarded as independent entities. The objective of this study is to investigate the topographical associations between the islet...

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Bibliographic Details
Published in:Clinical endocrinology (Oxford) 2008-09, Vol.69 (3), p.400-406
Main Authors: Zhao, Hai-Lu, Sui, Yi, Guan, Jing, Lai, Fernand M. M., Gu, Xue-Mei, He, Lan, Zhu, Xun, Rowlands, Dewi K., Xu, Gang, Tong, Peter C. Y., Chan, Juliana C. N.
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biological and medical sciences
Biomarkers - metabolism
Endocrine Cells - cytology
Endocrine Cells - immunology
Endocrine Cells - metabolism
Endocrinopathies
Epithelial Cells - cytology
Epithelial Cells - immunology
Epithelial Cells - metabolism
Female
Fundamental and applied biological sciences. Psychology
Glucagon - metabolism
Humans
Insulin - metabolism
Islets of Langerhans - cytology
Islets of Langerhans - immunology
Islets of Langerhans - metabolism
Male
Medical sciences
Middle Aged
Pancreas - cytology
Pancreas - immunology
Pancreas - metabolism
Pancreatic Ducts - cytology
Pancreatic Ducts - immunology
Pancreatic Ducts - metabolism
Vertebrates: endocrinology
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Summary:Summary Objectives  The pancreatic ducts, endocrine islets and exocrine acini are three functionally related components. From birth to adulthood, the islets and ducts are regarded as independent entities. The objective of this study is to investigate the topographical associations between the islet endocrine cells and duct epithelial cells in the adult human pancreas. Materials and methods  Panels of immunomarkers for the exocrine acinar cells (amylase), duct cells [cytokeratin 19 (CK19)], endocrine cells (chromogranin A, neurone specific enolase, synaptophysin) and islet hormones (glucagon, insulin, somatostatin, pancreatic polypeptide) were applied to sequential pancreatic tissue sections obtained from autopsy specimens of 10‐nondiabetic human adults. Double immunofluorescent staining with CK19 and islet hormones was performed to confirm the islet to duct interrelationship. Results  Sequential sectioning and immunostaining showed that 45% of the 172 islets examined appeared as single endocrine cell units or small clusters of
ISSN:0300-0664
1365-2265
DOI:10.1111/j.1365-2265.2008.03190.x