Enhanced expression of transgene in CHO cells using matrix attachment region

The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early prom...

Full description

Saved in:
Bibliographic Details
Published in:Cell biology international 2008-10, Vol.32 (10), p.1279-1283
Main Authors: Wang, Tian-Yun, Yang, Rui, Qin, Chuan, Wang, Li, Yang, Xian-Jun
Format: Article
Language:English
Subjects:
Animals
CHO Cells
Cricetinae
Cricetulus
Gene Dosage
Gene expression
Gene Expression Regulation
Gene silencing
Genes, Reporter
Genetic Vectors - genetics
Genetic Vectors - metabolism
Humans
Mammalian expression system
Matrix attachment region
Transfection
Transgenes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early promoter and flanked by two human β-globin MARs in cis. We transfected this vector into the Chinese hamster ovary (CHO) cell line, and found that the level of CAT gene expression with MAR was effectively increased, about 5.493-fold higher than those without MARs. Moreover, the variations of CAT expression among individuals of transformants were decreased 2.670-fold. Our result also showed that MAR could increase the proportion of positive colonies in recombinants.
ISSN:1065-6995
1095-8355
DOI:10.1016/j.cellbi.2008.07.014