Interferon gamma production during bovine respiratory syncytial virus (BRSV) infection is diminished in calves vaccinated with formalin-inactivated BRSV

• Published in: Vaccine 1999-03, Vol.17 (11), p.1293-1297
• Main Authors: Woolums, Amelia R., Singer, Randall S., Boyle, Gabrielle A., Gershwin, Laurel J.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Bovine respiratory syncytial virus; BRSV; Cattle; Cattle Diseases - metabolism; Cattle Diseases - prevention & control; Enzyme-Linked Immunosorbent Assay - veterinary; Formaldehyde; Formalin-inactivated RSV; Fundamental and applied biological sciences. Psychology; Interferon gamma; Interferon-gamma - biosynthesis; Interferon-gamma - blood; Leukocytes, Mononuclear - metabolism; Leukocytes, Mononuclear - virology; Male; Mice; Microbiology; Respiratory Syncytial Virus Infections - metabolism; Respiratory Syncytial Virus Infections - prevention & control; Respiratory Syncytial Virus Infections - veterinary; Respiratory Syncytial Virus, Bovine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Attenuated; Viral Vaccines; Virology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(98)00379-X

Formalin-inactivated respiratory syncytial virus (FI-RSV) vaccination has been associated with severe disease in humans. Research in mice suggests that FI-RSV may prime for decreased interferon gamma (IFN-γ) production at subsequent infection. Interferon-γ production by peripheral blood mononuclear cells (PBMC) was measured following challenge of calves vaccinated with FI-BRSV to determine whether a similar mechanism is operative in a host naturally susceptible to RSV. Eight-week old male Holstein calves were administered FI-BRSV and mock challenge (V/M, n=6); mock vaccination and BRSV challenge (M/C, n=6) or FI-BRSV and BRSV challenge (V/C, n=7). Vaccine was administered twice at a 2-week interval; challenge followed one month later. On days 0, 5 and 10 postchallenge (PC), PBMC were stimulated in vitro for 24 h with live BRSV, concanavalin A (positive control) or spent media (negative control). Supernatants were assayed for IFN-γ using ELISA. Interferon-γ production by BRSV-stimulated PBMC was increased in M/C and V/C calves as compared to V/M calves on day 5 PC ( p