A SEC-HPLC-ICP MS hyphenated technique for identification of sulfur-containing arsenic metabolites in biological samples

The present study describes the synthesis and characterization of thioarsenicals using electro-spray ionization-MS and time of flight-MS. Separation of thioarsenicals was found to be better by size-exclusion column compared to anion exchange column coupled with HPLC-inductively coupled argon plasma...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-10, Vol.874 (1), p.64-76
Main Authors: Mandal, Badal Kumar, Suzuki, Kazuo T., Anzai, Kazunori, Yamaguchi, Kentaro, Sei, Yoshihisa
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Arsenicals - chemistry
Arsenicals - metabolism
Arsenicals - urine
Biological and medical sciences
Cacodylic Acid - analogs & derivatives
Cacodylic Acid - metabolism
Cacodylic Acid - urine
Chromatography, High Pressure Liquid - methods
Dimethyldithioarsinic acid
Dimethyldithioarsinous acid
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Mass Spectrometry - methods
Medical sciences
Methylmonothioarsonous acid
Methyltrithioarsonic acid and biomolecules
Pharmacology. Drug treatments
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization
Sulfur - chemistry
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Summary:The present study describes the synthesis and characterization of thioarsenicals using electro-spray ionization-MS and time of flight-MS. Separation of thioarsenicals was found to be better by size-exclusion column compared to anion exchange column coupled with HPLC-inductively coupled argon plasma mass spectrometer (ICP MS). Although four thioarsenicals were confirmed as dimethylthioarsinous acid ( m/ z = 138), methylmonothioarsonous acid ( m/ z = 122), dimethyldithioarsinic acid ( m/ z = 170) and methyltrithioarsonic acid ( m/ z = 188), it is noted that HPLC-ICP MS alone were not sufficient for their identification. Also, none of them was stable with time. This is the first report detailing the synthesis and identification of methyltrithioarsonic acid. Both dimethyldithioarsinic acid and dimethylthioarsinous acid were detected in human nail samples while dimethyldithioarsinic acid was found in urine samples. So, the above technique could be applicable to the identification of sulfur-containing biomolecules in the biological samples.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.09.004