Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines

We have developed a chromatographic method suitable for the fractionation of polysaccharides having a negatively charged group. The method permits the removal of all those polysaccharide fragments having a short sequence and which are likely unsuitable for conjugate vaccine construction. The selecte...

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Bibliographic Details
Published in:Vaccine 1999-03, Vol.17 (9), p.1251-1263
Main Authors: Costantino, Paolo, Norelli, Francesco, Giannozzi, Aldo, D'Ascenzi, Sandro, Bartoloni, Antonella, Kaur, Surinder, Tang, Dazhi, Seid, Robert, Viti, Stefano, Paffetti, Roberto, Bigio, Massimo, Pennatini, Carlo, Averani, Giovanni, Guarnieri, Valentina, Gallo, Eugenia, Ravenscroft, Neil, Lazzeroni, Carla, Rappuoli, Rino, Ceccarini, Costante
Format: Article
Language:English
Subjects:
Antigens, Bacterial - chemistry
Bacterial Capsules - chemistry
Bacteriology
Biological and medical sciences
Capsular polysaccharide
Chromatography, Ion Exchange
Conjugate vaccines
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Haemophilus Vaccines - chemistry
Hydrolysis
Intermediate chain length oligomers
Mass Spectrometry
Microbiology
Neisseria meningitidis
Oligosaccharide
Polysaccharides, Bacterial - chemistry
Size fractionation
Ultrafiltration
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Conjugate - chemistry
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Summary:We have developed a chromatographic method suitable for the fractionation of polysaccharides having a negatively charged group. The method permits the removal of all those polysaccharide fragments having a short sequence and which are likely unsuitable for conjugate vaccine construction. The selected polysaccharide fragments can be used to produce glycoconjugate vaccines containing a restricted saccharide polydispersion. We have applied this chromatographic method to three different antigens, Haemophilus influenzae type b and Neisseria meningitidis group A and group C polysaccharides. The method is easily adapted for manufacturing purposes.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(98)00348-X