Focal Sites of DNA Repair Synthesis in Human Chromosomes

Nucleotide excision repair (NER) is the principle pathway by which the human cells eliminate UV-induced lesions from their genomic DNA. The process can be visualized through the labelling of the nucleotides that are incoporated into repair patches, following the excision of the damaged stretch of DN...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-04, Vol.257 (2), p.378-383
Main Authors: Svetlova, Maria P., Solovjeva, Liudmila V., Pleskach, Nadezhda A., Tomilin, Nikolai V.
Format: Article
Language:English
Subjects:
Biotin - analogs & derivatives
Cell Line
Cell Nucleus - genetics
Cell Nucleus - radiation effects
Chromosomes - genetics
Chromosomes - radiation effects
DNA - biosynthesis
DNA - genetics
DNA Damage - genetics
DNA Damage - radiation effects
DNA Repair - genetics
DNA Repair - radiation effects
DNA repair synthesis
Fibroblasts - metabolism
Fibroblasts - radiation effects
Fluorescent Antibody Technique
Fluorescent Dyes
focal sites
human fibroblasts
Humans
Idoxuridine - metabolism
immunofluorescence
Interphase
Metaphase
Skin
Software
Time Factors
Tyramine - analogs & derivatives
ultraviolet light
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nucleotide excision repair (NER) is the principle pathway by which the human cells eliminate UV-induced lesions from their genomic DNA. The process can be visualized through the labelling of the nucleotides that are incoporated into repair patches, following the excision of the damaged stretch of DNA. In this study we have visualized sites of DNA repair synthesis (DRS) in human interphase and metaphase chromosomes after very short times (2.5–30 min) of postirradiation labellingin vivowith 5-iododeoxyuridine. A limited number (
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0485