A 1.5-Mb Contig within the Cat Eye Syndrome Critical Region at Human Chromosome 22q11.2

We have constructed a 1.5-Mb contig spanning the distal half of the critical region for cat eye syndrome on human chromosome 22 from D22S543 to D22S181. The contig consists of 20 P1 artificial chromosome (PAC) clones and 11 bacterial artificial chromosome (BAC) clones screened from 2 BAC and 2 PAC l...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1999-04, Vol.57 (2), p.306-309
Main Authors: Johnson, Angela, Minoshima, Shinsei, Asakawa, Shuichi, Shimizu, Nobuyoshi, Shizuya, Hiroaki, Roe, Bruce A., McDermid, Heather E.
Format: Article
Language:English
Subjects:
Abnormalities, Multiple - genetics
Bacteria - genetics
Bacteriophage P1 - genetics
Biological and medical sciences
Chromosomes, Human, Pair 22 - genetics
Coloboma - genetics
Complex syndromes
Contig Mapping
DNA - genetics
Electrophoresis, Gel, Pulsed-Field
Genetic Markers
Humans
Medical genetics
Medical sciences
Restriction Mapping
Syndrome
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Summary:We have constructed a 1.5-Mb contig spanning the distal half of the critical region for cat eye syndrome on human chromosome 22 from D22S543 to D22S181. The contig consists of 20 P1 artificial chromosome (PAC) clones and 11 bacterial artificial chromosome (BAC) clones screened from 2 BAC and 2 PAC libraries. Continuous overlap between the clones was confirmed using vectorette PCR and riboprobes. Despite the instability of this region in a previous YAC contig, only 1 BAC showed a minor instability and then in only one isolation. This contig is now providing the basis for genomic sequencing and gene identification in the cat eye syndrome critical region.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1999.5757