Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1

Porcine parvovirus (PPV) infection is associated with reproductive losses in swine and its causative agent, the PPV, has been isolated worldwide. Serological surveys and virus isolation studies throughout Brazil confirm the occurrence of PPV infection in this country. The most common methods to dete...

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Bibliographic Details
Published in:Journal of virological methods 1999-03, Vol.78 (1), p.191-198
Main Authors: Soares, Rodrigo M., Durigon, Edison L., Bersano, Josete G., Richtzenhain, Leonardo J.
Format: Article
Language:English
Subjects:
Abortion, Veterinary - virology
Animals
Base Sequence
Biological and medical sciences
Cell Line
Detection of viral DNA
DNA Primers - genetics
DNA, Viral - analysis
Female
Fetal Death - veterinary
Fundamental and applied biological sciences. Psychology
Kidney - cytology
Microbiology
Molecular Sequence Data
Parvoviridae Infections - veterinary
Parvoviridae Infections - virology
Parvovirus - genetics
Parvovirus - isolation & purification
Polymerase chain reaction
Polymerase Chain Reaction - methods
Porcine parvovirus
Pregnancy
Swine
Swine Diseases - virology
Techniques used in virology
Viral Nonstructural Proteins - genetics
Virology
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Summary:Porcine parvovirus (PPV) infection is associated with reproductive losses in swine and its causative agent, the PPV, has been isolated worldwide. Serological surveys and virus isolation studies throughout Brazil confirm the occurrence of PPV infection in this country. The most common methods to detect PPV infection are fluorescent antibody staining of fetal tissues, hemagglutination assay of tissue extracts and virus isolation from fetal tissues. Non-specificity and low sensitivity are the major drawbacks of these techniques. The development of a polymerase chain reaction (PCR) and nested-PCR assays for PPV DNA detection from infected cell lines and clinical samples is described. The primers were designed to a highly conserved region of the PPV genome which codes for the non-structural protein, NS-1. Results showed that PCR could detect PPV in titres at least 10 6 higher than the hemagglutination assay. The PCR and nested-PCR assays were used to detect successfully PPV DNA in clinical samples.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(98)00177-3