Factors Associated with Peripheral Blood Stem Cell Yield in Volunteer Donors Mobilized with Granulocyte Colony-Stimulating Factors: The Impact of Donor Characteristics and Procedural Settings

• Published in: Biology of blood and marrow transplantation 2008-11, Vol.14 (11), p.1305-1311
• Main Authors: Wang, Tso-Fu, Wen, Shu-Hui, Chen, Rong-Long, Lu, Chi-Jui, Zheng, Ya-Jun, Yang, Shang-Hsien, Chu, Sung-Chao, Kao, Ruey-Ho, Chen, Shu-Huey
• Format: Article
• Language: English
• Subjects: Adult; Antigens, CD34; Apheresis; Body Mass Index; Cell Separation; Female; Granulocyte colony-stimulating factor; Granulocyte Colony-Stimulating Factor - administration & dosage; Healthy donor; Hematology, Oncology and Palliative Medicine; Hematopoietic Stem Cell Mobilization; Hematopoietic Stem Cells; Humans; Leukocyte Count; Living Donors; Male; Sex Characteristics; Sex Factors
• ISSN: 1083-8791; 1523-6536
• DOI: 10.1016/j.bbmt.2008.09.002

Peripheral blood stem cells (PBSCs) are increasingly used as the source of hematopoietic stem cells, but there are large variations in harvest outcome between individuals mobilized by granulocyte colony-stimulating factor (G-CSF). We examined the effects of donor characteristics and procedure factors on the day 1 CD34+ cell yield in 373 unrelated healthy donors. G-CSF was administered subcutaneously at a planned dose of 8.3 to 11μg/kg daily for 5 days, followed by harvest started on day 5 of G-CSF treatment. Of the 373 donors, 159 (42.6%) had the radial artery as the inlet access for harvest. Poor day 1 cell yield was defined as < 10 × 106 CD34+ cells/L of processed blood for the first apheresis; 62 donors (16.6%) did not attain this threshold. The male donors had significantly higher yields at harvest compared with the female donors. The female donors had higher CD34+ cell yields if the circulation access was through an artery than if is was through a vein. In a multiple regression analysis, donor age, sex, body mass index (BMI), preharvest white blood cell and circulating immature cell counts, access type, and flow rate correlated with day 1 yield. Female sex, older age, venous access, and a higher flow rate were significantly associated with greater risk for a day 1 poor yield of CD34+ cells (odds ratio = 3.0074, 1.045, 4.3362, and 1.1131, respectively). A higher BMI may decrease the risk (odds ratio = 0.8472). In donors at higher risk for poor CD34+ cell yield, strategies for increasing CD34+ cells must be considered.