Apoptotic Human Lymphocytes Have Diminished CD4 and CD8 Receptor Expression

We used quantitative multiparameter flow cytometric assays to simultaneously detect viable, apoptotic, and necrotic human peripheral blood mononuclear cells (PBMC) and immunophenotyped lymphocyte subsets within the PBMC. Apoptosis was induced by a spectrum of treatments, including camptothecin, cisp...

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Bibliographic Details
Published in:Cellular immunology 1999-04, Vol.193 (1), p.36-47
Main Authors: Potter, Alan, Kim, Christine, Gollahon, Katherine A., Rabinovitch, Peter S.
Format: Article
Language:English
Subjects:
Apoptosis
CD3 Complex - immunology
CD4 Antigens - analysis
CD8 Antigens - analysis
Cycloheximide - pharmacology
Etoposide - pharmacology
Humans
Lymphocytes - chemistry
Lymphocytes - physiology
Necrosis
Transglutaminases - metabolism
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Summary:We used quantitative multiparameter flow cytometric assays to simultaneously detect viable, apoptotic, and necrotic human peripheral blood mononuclear cells (PBMC) and immunophenotyped lymphocyte subsets within the PBMC. Apoptosis was induced by a spectrum of treatments, including camptothecin, cisplatin, dexamethasone, hyperthermia, staurosporine, and etoposide in anti-CD3 mAb-stimulated cells and by cyclohexamide in both quiescent and stimulated cells; apoptosis in the latter was augmented by anti-fas mAb. We found that CD4+and CD8+cells were significantly underrepresented in the apoptotic PBMC and that the percentage of CD4+and CD8+PBMC each markedly decreased as apoptosis increased. This suggested that surface expression of these receptors was lessened on apoptotic CD4+and CD8+cells. This was directly confirmed by observation of sorted CD4+PBMC. This analysis of a wide variety of apoptotic stimuli demonstrates that diminished CD4 and CD8 surface receptor expression is a common feature of human T lymphocyte apoptosis.
ISSN:0008-8749
1090-2163
DOI:10.1006/cimm.1998.1443