Loading…
Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis subsp. nov., a pathogen of the oyster Crassostrea gigas
Eleven Vibrio isolates invading the hemolymph of live and moribund oysters ( Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains a...
Saved in:
| Published in: | Systematic and applied microbiology 2008-10, Vol.31 (5), p.358-365 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c553t-be60e9d55d9123c16fb581a8f5de626f360e3bae13bd628c50a662052c39d3f63 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c553t-be60e9d55d9123c16fb581a8f5de626f360e3bae13bd628c50a662052c39d3f63 |
| container_end_page | 365 |
| container_issue | 5 |
| container_start_page | 358 |
| container_title | Systematic and applied microbiology |
| container_volume | 31 |
| creator | Garnier, Matthieu Labreuche, Yannick Nicolas, Jean-Louis |
| description | Eleven
Vibrio isolates invading the hemolymph of live and moribund oysters (
Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA,
gyrB and
toxR genes indicated high homogeneity between these strains and the
Vibrio aestuarianus type strain (ATCC35048
T), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the
V. aestuarianus spp. group,
V. aestuarianus subsp.
aestuarianus [D. Tison, R. Seidler,
Vibrio aestuarianus: a new species from estuarine waters and shellfish, Int. J. Syst. Bacteriol. (1983) 699–702] and
V. aestuarianus subsp.
francensis for these French isolates. The characters that differentiate the new strains from
V. aestuarianus subsp.
aestuarianus
T are virulence (positive for 63% of the isolates) and 12:0 fatty acid content. The colonies were smaller and uncoloured, whereas no growth occurred at 35
°C or on TCBS, and the strains did not utilize several substrates, including
l-serine,
α-cyclodextrin,
d-mannitol,
α-glycyl-
l-aspartic acid,
l-threonine and glucose-1-phosphate. |
| doi_str_mv | 10.1016/j.syapm.2008.06.003 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69696800</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S072320200800057X</els_id><sourcerecordid>19394577</sourcerecordid><originalsourceid>FETCH-LOGICAL-c553t-be60e9d55d9123c16fb581a8f5de626f360e3bae13bd628c50a662052c39d3f63</originalsourceid><addsrcrecordid>eNqFkcGO0zAQhi0EYruFJ0ACX-BEwthunOSwB1SxgLSIAyxXa-I4ras0Dp5kpfIIPDXetlpuII9kyfPNr_H_M_ZCQC5A6He7nA447nMJUOWgcwD1iC2EFlUGdbV6zBZQSpVJkHDBLol2AGJVa_GUXYiqTKVgwX5_Cb2zc4-R49DyceuGMB1Gb7ndYkQ7ueh_4eTDwEPHf_gm-sDR0TRj9DjMxGluaMx5F3GwbiD_8DKEu_wtRz7itA0bdxSYto6HAyVVvo5IFGiKDvnGb5CesScd9uSen-8lu73-8H39Kbv5-vHz-v1NZotCTVnjNLi6LYq2FlJZobumqARWXdE6LXWnUls16IRqWi0rWwBqLaGQVtWt6rRasjcn3TGGn3P6itl7sq7vcXBhJqPrdKrk5v9AUat6VZRlAtUJtDEQRdeZMfo9xoMRYO6zMjtzzMrcZ2VAm6Sepl6e5edm79q_M-dwEvD6DCBZ7I8Oe3rgJJR1KdISS_bqxHUYDG5iYm6_SRAKRFGXshKJuDoRLvl65100ZL1LebU-OjuZNvh_rvoHJ4S--g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19394577</pqid></control><display><type>article</type><title>Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis subsp. nov., a pathogen of the oyster Crassostrea gigas</title><source>Elsevier</source><creator>Garnier, Matthieu ; Labreuche, Yannick ; Nicolas, Jean-Louis</creator><creatorcontrib>Garnier, Matthieu ; Labreuche, Yannick ; Nicolas, Jean-Louis</creatorcontrib><description>Eleven
Vibrio isolates invading the hemolymph of live and moribund oysters (
Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA,
gyrB and
toxR genes indicated high homogeneity between these strains and the
Vibrio aestuarianus type strain (ATCC35048
T), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the
V. aestuarianus spp. group,
V. aestuarianus subsp.
aestuarianus [D. Tison, R. Seidler,
Vibrio aestuarianus: a new species from estuarine waters and shellfish, Int. J. Syst. Bacteriol. (1983) 699–702] and
V. aestuarianus subsp.
francensis for these French isolates. The characters that differentiate the new strains from
V. aestuarianus subsp.
aestuarianus
T are virulence (positive for 63% of the isolates) and 12:0 fatty acid content. The colonies were smaller and uncoloured, whereas no growth occurred at 35
°C or on TCBS, and the strains did not utilize several substrates, including
l-serine,
α-cyclodextrin,
d-mannitol,
α-glycyl-
l-aspartic acid,
l-threonine and glucose-1-phosphate.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2008.06.003</identifier><identifier>PMID: 18718730</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>München: Elsevier GmbH</publisher><subject>animal pathogenic bacteria ; Animals ; Bacterial Typing Techniques - methods ; Bacteriology ; Biological and medical sciences ; Crassostrea - microbiology ; Crassostrea gigas ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; France ; French isolates ; Fundamental and applied biological sciences. Psychology ; Genotype ; Hemolymph - microbiology ; Marine ; Microbiology ; molecular systematics ; new geographic records ; new species ; Nucleic Acid Hybridization ; oysters ; Pathogen ; Phenotype ; phenotypic variation ; Phylogeny ; Polyphasic approach ; RNA, Ribosomal, 16S - genetics ; Species Specificity ; Summer mortalities ; Systematics ; Vibrio ; Vibrio - classification ; Vibrio - genetics ; Vibrio - isolation & purification ; Vibrio - pathogenicity ; Vibrio aestuarianus ; Vibrio aestuarianus subsp. francensis ; Virulence</subject><ispartof>Systematic and applied microbiology, 2008-10, Vol.31 (5), p.358-365</ispartof><rights>2008 Elsevier GmbH</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c553t-be60e9d55d9123c16fb581a8f5de626f360e3bae13bd628c50a662052c39d3f63</citedby><cites>FETCH-LOGICAL-c553t-be60e9d55d9123c16fb581a8f5de626f360e3bae13bd628c50a662052c39d3f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20797119$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18718730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garnier, Matthieu</creatorcontrib><creatorcontrib>Labreuche, Yannick</creatorcontrib><creatorcontrib>Nicolas, Jean-Louis</creatorcontrib><title>Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis subsp. nov., a pathogen of the oyster Crassostrea gigas</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Eleven
Vibrio isolates invading the hemolymph of live and moribund oysters (
Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA,
gyrB and
toxR genes indicated high homogeneity between these strains and the
Vibrio aestuarianus type strain (ATCC35048
T), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the
V. aestuarianus spp. group,
V. aestuarianus subsp.
aestuarianus [D. Tison, R. Seidler,
Vibrio aestuarianus: a new species from estuarine waters and shellfish, Int. J. Syst. Bacteriol. (1983) 699–702] and
V. aestuarianus subsp.
francensis for these French isolates. The characters that differentiate the new strains from
V. aestuarianus subsp.
aestuarianus
T are virulence (positive for 63% of the isolates) and 12:0 fatty acid content. The colonies were smaller and uncoloured, whereas no growth occurred at 35
°C or on TCBS, and the strains did not utilize several substrates, including
l-serine,
α-cyclodextrin,
d-mannitol,
α-glycyl-
l-aspartic acid,
l-threonine and glucose-1-phosphate.</description><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Crassostrea - microbiology</subject><subject>Crassostrea gigas</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>France</subject><subject>French isolates</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Hemolymph - microbiology</subject><subject>Marine</subject><subject>Microbiology</subject><subject>molecular systematics</subject><subject>new geographic records</subject><subject>new species</subject><subject>Nucleic Acid Hybridization</subject><subject>oysters</subject><subject>Pathogen</subject><subject>Phenotype</subject><subject>phenotypic variation</subject><subject>Phylogeny</subject><subject>Polyphasic approach</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Species Specificity</subject><subject>Summer mortalities</subject><subject>Systematics</subject><subject>Vibrio</subject><subject>Vibrio - classification</subject><subject>Vibrio - genetics</subject><subject>Vibrio - isolation & purification</subject><subject>Vibrio - pathogenicity</subject><subject>Vibrio aestuarianus</subject><subject>Vibrio aestuarianus subsp. francensis</subject><subject>Virulence</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkcGO0zAQhi0EYruFJ0ACX-BEwthunOSwB1SxgLSIAyxXa-I4ras0Dp5kpfIIPDXetlpuII9kyfPNr_H_M_ZCQC5A6He7nA447nMJUOWgcwD1iC2EFlUGdbV6zBZQSpVJkHDBLol2AGJVa_GUXYiqTKVgwX5_Cb2zc4-R49DyceuGMB1Gb7ndYkQ7ueh_4eTDwEPHf_gm-sDR0TRj9DjMxGluaMx5F3GwbiD_8DKEu_wtRz7itA0bdxSYto6HAyVVvo5IFGiKDvnGb5CesScd9uSen-8lu73-8H39Kbv5-vHz-v1NZotCTVnjNLi6LYq2FlJZobumqARWXdE6LXWnUls16IRqWi0rWwBqLaGQVtWt6rRasjcn3TGGn3P6itl7sq7vcXBhJqPrdKrk5v9AUat6VZRlAtUJtDEQRdeZMfo9xoMRYO6zMjtzzMrcZ2VAm6Sepl6e5edm79q_M-dwEvD6DCBZ7I8Oe3rgJJR1KdISS_bqxHUYDG5iYm6_SRAKRFGXshKJuDoRLvl65100ZL1LebU-OjuZNvh_rvoHJ4S--g</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Garnier, Matthieu</creator><creator>Labreuche, Yannick</creator><creator>Nicolas, Jean-Louis</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis subsp. nov., a pathogen of the oyster Crassostrea gigas</title><author>Garnier, Matthieu ; Labreuche, Yannick ; Nicolas, Jean-Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c553t-be60e9d55d9123c16fb581a8f5de626f360e3bae13bd628c50a662052c39d3f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Crassostrea - microbiology</topic><topic>Crassostrea gigas</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>France</topic><topic>French isolates</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Hemolymph - microbiology</topic><topic>Marine</topic><topic>Microbiology</topic><topic>molecular systematics</topic><topic>new geographic records</topic><topic>new species</topic><topic>Nucleic Acid Hybridization</topic><topic>oysters</topic><topic>Pathogen</topic><topic>Phenotype</topic><topic>phenotypic variation</topic><topic>Phylogeny</topic><topic>Polyphasic approach</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Species Specificity</topic><topic>Summer mortalities</topic><topic>Systematics</topic><topic>Vibrio</topic><topic>Vibrio - classification</topic><topic>Vibrio - genetics</topic><topic>Vibrio - isolation & purification</topic><topic>Vibrio - pathogenicity</topic><topic>Vibrio aestuarianus</topic><topic>Vibrio aestuarianus subsp. francensis</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garnier, Matthieu</creatorcontrib><creatorcontrib>Labreuche, Yannick</creatorcontrib><creatorcontrib>Nicolas, Jean-Louis</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garnier, Matthieu</au><au>Labreuche, Yannick</au><au>Nicolas, Jean-Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis subsp. nov., a pathogen of the oyster Crassostrea gigas</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>31</volume><issue>5</issue><spage>358</spage><epage>365</epage><pages>358-365</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>Eleven
Vibrio isolates invading the hemolymph of live and moribund oysters (
Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA,
gyrB and
toxR genes indicated high homogeneity between these strains and the
Vibrio aestuarianus type strain (ATCC35048
T), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the
V. aestuarianus spp. group,
V. aestuarianus subsp.
aestuarianus [D. Tison, R. Seidler,
Vibrio aestuarianus: a new species from estuarine waters and shellfish, Int. J. Syst. Bacteriol. (1983) 699–702] and
V. aestuarianus subsp.
francensis for these French isolates. The characters that differentiate the new strains from
V. aestuarianus subsp.
aestuarianus
T are virulence (positive for 63% of the isolates) and 12:0 fatty acid content. The colonies were smaller and uncoloured, whereas no growth occurred at 35
°C or on TCBS, and the strains did not utilize several substrates, including
l-serine,
α-cyclodextrin,
d-mannitol,
α-glycyl-
l-aspartic acid,
l-threonine and glucose-1-phosphate.</abstract><cop>München</cop><pub>Elsevier GmbH</pub><pmid>18718730</pmid><doi>10.1016/j.syapm.2008.06.003</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0723-2020 |
| ispartof | Systematic and applied microbiology, 2008-10, Vol.31 (5), p.358-365 |
| issn | 0723-2020 1618-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69696800 |
| source | Elsevier |
| subjects | animal pathogenic bacteria Animals Bacterial Typing Techniques - methods Bacteriology Biological and medical sciences Crassostrea - microbiology Crassostrea gigas DNA, Bacterial - analysis DNA, Bacterial - genetics France French isolates Fundamental and applied biological sciences. Psychology Genotype Hemolymph - microbiology Marine Microbiology molecular systematics new geographic records new species Nucleic Acid Hybridization oysters Pathogen Phenotype phenotypic variation Phylogeny Polyphasic approach RNA, Ribosomal, 16S - genetics Species Specificity Summer mortalities Systematics Vibrio Vibrio - classification Vibrio - genetics Vibrio - isolation & purification Vibrio - pathogenicity Vibrio aestuarianus Vibrio aestuarianus subsp. francensis Virulence |
| title | Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis subsp. nov., a pathogen of the oyster Crassostrea gigas |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T12%3A53%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20and%20phenotypic%20characterization%20of%20Vibrio%20aestuarianus%20subsp.%20francensis%20subsp.%20nov.,%20a%20pathogen%20of%20the%20oyster%20Crassostrea%20gigas&rft.jtitle=Systematic%20and%20applied%20microbiology&rft.au=Garnier,%20Matthieu&rft.date=2008-10-01&rft.volume=31&rft.issue=5&rft.spage=358&rft.epage=365&rft.pages=358-365&rft.issn=0723-2020&rft.eissn=1618-0984&rft.coden=SAMIDF&rft_id=info:doi/10.1016/j.syapm.2008.06.003&rft_dat=%3Cproquest_cross%3E19394577%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c553t-be60e9d55d9123c16fb581a8f5de626f360e3bae13bd628c50a662052c39d3f63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=19394577&rft_id=info:pmid/18718730&rfr_iscdi=true |