Leptin Up-Regulates the Lactogenic Effect of Prolactin in the Bovine Mammary Gland In Vitro

The ability of leptin to up-regulate prolactin action in the mammary gland is well established. We examined the effect of leptin and prolactin on traits associated with lactation. Leptin and prolactin enhanced proliferation (thymidine incorporation) of the mammary gland cells, elevated the cells’ pr...

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Bibliographic Details
Published in:Journal of dairy science 2008-11, Vol.91 (11), p.4183-4189
Main Authors: Feuermann, Y., Shamay, A., Mabjeesh, S.J.
Format: Article
Language:English
Subjects:
amino acid metabolism
aminopeptidase N/CD13
Animal productions
Animals
apoptosis
Apoptosis - drug effects
biochemical pathways
Biological and medical sciences
Cattle
CD13 Antigens - metabolism
cell proliferation
Cell Proliferation - drug effects
cows
dosage
dose response
Female
Food industries
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic - drug effects
in vitro studies
lactation
leptin
Leptin - pharmacology
mammary glands
Mammary Glands, Animal - cytology
Mammary Glands, Animal - drug effects
Mammary Glands, Animal - enzymology
Milk and cheese industries. Ice creams
milk synthesis
prolactin
Prolactin - pharmacology
Protein Kinases - metabolism
protein synthesis
signal transduction
synergism
Terrestrial animal productions
tissue culture
TOR Serine-Threonine Kinases
Up-Regulation - drug effects
Vertebrates
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Summary:The ability of leptin to up-regulate prolactin action in the mammary gland is well established. We examined the effect of leptin and prolactin on traits associated with lactation. Leptin and prolactin enhanced proliferation (thymidine incorporation) of the mammary gland cells, elevated the cells’ proliferation in a dose-responsive manner, and synergized to elevate the expression of amino acid metabolism via a 90% increase in aminopeptidase N expression. Leptin and prolactin decreased apoptosis (decreased caspase-3 expression by 60%) in the same manner. Leptin enhanced the effect of prolactin on all of these processes in bovine mammary explants. Leptin and prolactin regulated mTOR (mammalian target of rapamycin) by increasing expression by 66%, which is one of the signal-transduction junctions involved in the regulation of proliferation, apoptosis, and protein synthesis. These findings support the hypothesis that leptin up-regulates prolactin action in the bovine mammary gland.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2008-0988