Loading…
Effects of serotonin on neurite outgrowth from thalamic neurons in vitro
Altering levels of serotonin in the primary somatosensory cortex during early postnatal life influences thalamocortical development. Recent in vivo experiments suggest that serotonin may have direct effects on the growth of thalamocortical axons, and the present study was undertaken to determine whe...
Saved in:
| Published in: | Neuroscience 1999-05, Vol.90 (3), p.967-974 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c456t-f271d1e6ef66ebdef71d81571b0a008140a3aff3587395461a3cbbe045d1754f3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c456t-f271d1e6ef66ebdef71d81571b0a008140a3aff3587395461a3cbbe045d1754f3 |
| container_end_page | 974 |
| container_issue | 3 |
| container_start_page | 967 |
| container_title | Neuroscience |
| container_volume | 90 |
| creator | Lieske, V. Bennett-Clarke, C.A. Rhoades, R.W. |
| description | Altering levels of serotonin in the primary somatosensory cortex during early postnatal life influences thalamocortical development. Recent
in vivo experiments suggest that serotonin may have direct effects on the growth of thalamocortical axons, and the present study was undertaken to determine whether this amine influences process outgrowth from thalamic cells maintained in culture. Ventrobasal thalamic neurons were harvested from newborn rats and maintained in culture for eight days. At the end of this period, 0, 10, 25, 50 or 100
μM serotonin was added to the culture medium. After an additional six days, cultures were fixed and stained with neuron-specific enolase. Quantitative analysis of >500 cells from each condition indicated that 25
μM serotonin, but not the other concentrations of this amine, significantly increased the length of the primary (longest) process growing out from the cell body (
P |
| doi_str_mv | 10.1016/S0306-4522(98)00501-6 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69713366</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0306452298005016</els_id><sourcerecordid>69713366</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-f271d1e6ef66ebdef71d81571b0a008140a3aff3587395461a3cbbe045d1754f3</originalsourceid><addsrcrecordid>eNqFkE1LxDAQhoMouq7-BKUHET1Uk81H25PIsrqC4EE9hzSdaKRt1iRV_PdmP1BvziUMeSaT90HoiOALgom4fMQUi5zxyeSsKs8x5pjkYguNSFnQvOCMbaPRD7KH9kN4w6k4o7toj-BJ4ioxQvOZMaBjyJzJAngXXW_7zPVZD4O3ETI3xBfvPuNrZrzrsviqWtVZvbp3fcgS_WGjdwdox6g2wOHmHKPnm9nTdJ7fP9zeTa_vc824iLmZFKQhIMAIAXUDJrUl4QWpscK4JAwrqoyhPMWoOBNEUV3XgBlvSEpl6Bidrt9dePc-QIiys0FD26oe3BCkqApCqRAJ5GtQexeCByMX3nbKf0mC5VKhXCmUSz-yKuVKoVzOHW8WDHUHzZ-ptbMEnGwAFbRqjVe9tuGXK1jFKp6wqzUGycaHBS-DttBraKxPxmXj7D8_-QYU8o1W</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69713366</pqid></control><display><type>article</type><title>Effects of serotonin on neurite outgrowth from thalamic neurons in vitro</title><source>ScienceDirect Journals</source><creator>Lieske, V. ; Bennett-Clarke, C.A. ; Rhoades, R.W.</creator><creatorcontrib>Lieske, V. ; Bennett-Clarke, C.A. ; Rhoades, R.W.</creatorcontrib><description>Altering levels of serotonin in the primary somatosensory cortex during early postnatal life influences thalamocortical development. Recent
in vivo experiments suggest that serotonin may have direct effects on the growth of thalamocortical axons, and the present study was undertaken to determine whether this amine influences process outgrowth from thalamic cells maintained in culture. Ventrobasal thalamic neurons were harvested from newborn rats and maintained in culture for eight days. At the end of this period, 0, 10, 25, 50 or 100
μM serotonin was added to the culture medium. After an additional six days, cultures were fixed and stained with neuron-specific enolase. Quantitative analysis of >500 cells from each condition indicated that 25
μM serotonin, but not the other concentrations of this amine, significantly increased the length of the primary (longest) process growing out from the cell body (
P<0.001), the total (summed) length of all processes (
P<0.0001), total neurites per cell (
P<0.05), number of branch points per cell (
P<0.01) and branch points on the primary neurite (
P<0.0005).
These results demonstrate that exposing thalamic cells to serotonin increases process outgrowth from them in the absence of their cortical targets.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/S0306-4522(98)00501-6</identifier><identifier>PMID: 10218796</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>5-HT 1B receptor ; 5-HT transporter ; Animals ; Animals, Newborn - physiology ; barrel ; Biological and medical sciences ; Development. Senescence. Regeneration. Transplantation ; Fundamental and applied biological sciences. Psychology ; Neurites - drug effects ; Neurites - physiology ; Neurons - drug effects ; Neurons - physiology ; Rats ; Rats, Sprague-Dawley ; Reference Values ; serotonin ; Serotonin - pharmacology ; thalamocortical axon ; Thalamus - cytology ; Thalamus - drug effects ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 1999-05, Vol.90 (3), p.967-974</ispartof><rights>1999 IBRO</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-f271d1e6ef66ebdef71d81571b0a008140a3aff3587395461a3cbbe045d1754f3</citedby><cites>FETCH-LOGICAL-c456t-f271d1e6ef66ebdef71d81571b0a008140a3aff3587395461a3cbbe045d1754f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1749495$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10218796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lieske, V.</creatorcontrib><creatorcontrib>Bennett-Clarke, C.A.</creatorcontrib><creatorcontrib>Rhoades, R.W.</creatorcontrib><title>Effects of serotonin on neurite outgrowth from thalamic neurons in vitro</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Altering levels of serotonin in the primary somatosensory cortex during early postnatal life influences thalamocortical development. Recent
in vivo experiments suggest that serotonin may have direct effects on the growth of thalamocortical axons, and the present study was undertaken to determine whether this amine influences process outgrowth from thalamic cells maintained in culture. Ventrobasal thalamic neurons were harvested from newborn rats and maintained in culture for eight days. At the end of this period, 0, 10, 25, 50 or 100
μM serotonin was added to the culture medium. After an additional six days, cultures were fixed and stained with neuron-specific enolase. Quantitative analysis of >500 cells from each condition indicated that 25
μM serotonin, but not the other concentrations of this amine, significantly increased the length of the primary (longest) process growing out from the cell body (
P<0.001), the total (summed) length of all processes (
P<0.0001), total neurites per cell (
P<0.05), number of branch points per cell (
P<0.01) and branch points on the primary neurite (
P<0.0005).
These results demonstrate that exposing thalamic cells to serotonin increases process outgrowth from them in the absence of their cortical targets.</description><subject>5-HT 1B receptor</subject><subject>5-HT transporter</subject><subject>Animals</subject><subject>Animals, Newborn - physiology</subject><subject>barrel</subject><subject>Biological and medical sciences</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Neurites - drug effects</subject><subject>Neurites - physiology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Values</subject><subject>serotonin</subject><subject>Serotonin - pharmacology</subject><subject>thalamocortical axon</subject><subject>Thalamus - cytology</subject><subject>Thalamus - drug effects</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAQhoMouq7-BKUHET1Uk81H25PIsrqC4EE9hzSdaKRt1iRV_PdmP1BvziUMeSaT90HoiOALgom4fMQUi5zxyeSsKs8x5pjkYguNSFnQvOCMbaPRD7KH9kN4w6k4o7toj-BJ4ioxQvOZMaBjyJzJAngXXW_7zPVZD4O3ETI3xBfvPuNrZrzrsviqWtVZvbp3fcgS_WGjdwdox6g2wOHmHKPnm9nTdJ7fP9zeTa_vc824iLmZFKQhIMAIAXUDJrUl4QWpscK4JAwrqoyhPMWoOBNEUV3XgBlvSEpl6Bidrt9dePc-QIiys0FD26oe3BCkqApCqRAJ5GtQexeCByMX3nbKf0mC5VKhXCmUSz-yKuVKoVzOHW8WDHUHzZ-ptbMEnGwAFbRqjVe9tuGXK1jFKp6wqzUGycaHBS-DttBraKxPxmXj7D8_-QYU8o1W</recordid><startdate>199905</startdate><enddate>199905</enddate><creator>Lieske, V.</creator><creator>Bennett-Clarke, C.A.</creator><creator>Rhoades, R.W.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199905</creationdate><title>Effects of serotonin on neurite outgrowth from thalamic neurons in vitro</title><author>Lieske, V. ; Bennett-Clarke, C.A. ; Rhoades, R.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-f271d1e6ef66ebdef71d81571b0a008140a3aff3587395461a3cbbe045d1754f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>5-HT 1B receptor</topic><topic>5-HT transporter</topic><topic>Animals</topic><topic>Animals, Newborn - physiology</topic><topic>barrel</topic><topic>Biological and medical sciences</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Neurites - drug effects</topic><topic>Neurites - physiology</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Values</topic><topic>serotonin</topic><topic>Serotonin - pharmacology</topic><topic>thalamocortical axon</topic><topic>Thalamus - cytology</topic><topic>Thalamus - drug effects</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lieske, V.</creatorcontrib><creatorcontrib>Bennett-Clarke, C.A.</creatorcontrib><creatorcontrib>Rhoades, R.W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lieske, V.</au><au>Bennett-Clarke, C.A.</au><au>Rhoades, R.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of serotonin on neurite outgrowth from thalamic neurons in vitro</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1999-05</date><risdate>1999</risdate><volume>90</volume><issue>3</issue><spage>967</spage><epage>974</epage><pages>967-974</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Altering levels of serotonin in the primary somatosensory cortex during early postnatal life influences thalamocortical development. Recent
in vivo experiments suggest that serotonin may have direct effects on the growth of thalamocortical axons, and the present study was undertaken to determine whether this amine influences process outgrowth from thalamic cells maintained in culture. Ventrobasal thalamic neurons were harvested from newborn rats and maintained in culture for eight days. At the end of this period, 0, 10, 25, 50 or 100
μM serotonin was added to the culture medium. After an additional six days, cultures were fixed and stained with neuron-specific enolase. Quantitative analysis of >500 cells from each condition indicated that 25
μM serotonin, but not the other concentrations of this amine, significantly increased the length of the primary (longest) process growing out from the cell body (
P<0.001), the total (summed) length of all processes (
P<0.0001), total neurites per cell (
P<0.05), number of branch points per cell (
P<0.01) and branch points on the primary neurite (
P<0.0005).
These results demonstrate that exposing thalamic cells to serotonin increases process outgrowth from them in the absence of their cortical targets.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10218796</pmid><doi>10.1016/S0306-4522(98)00501-6</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0306-4522 |
| ispartof | Neuroscience, 1999-05, Vol.90 (3), p.967-974 |
| issn | 0306-4522 1873-7544 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69713366 |
| source | ScienceDirect Journals |
| subjects | 5-HT 1B receptor 5-HT transporter Animals Animals, Newborn - physiology barrel Biological and medical sciences Development. Senescence. Regeneration. Transplantation Fundamental and applied biological sciences. Psychology Neurites - drug effects Neurites - physiology Neurons - drug effects Neurons - physiology Rats Rats, Sprague-Dawley Reference Values serotonin Serotonin - pharmacology thalamocortical axon Thalamus - cytology Thalamus - drug effects Vertebrates: nervous system and sense organs |
| title | Effects of serotonin on neurite outgrowth from thalamic neurons in vitro |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T22%3A24%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20serotonin%20on%20neurite%20outgrowth%20from%20thalamic%20neurons%20in%20vitro&rft.jtitle=Neuroscience&rft.au=Lieske,%20V.&rft.date=1999-05&rft.volume=90&rft.issue=3&rft.spage=967&rft.epage=974&rft.pages=967-974&rft.issn=0306-4522&rft.eissn=1873-7544&rft.coden=NRSCDN&rft_id=info:doi/10.1016/S0306-4522(98)00501-6&rft_dat=%3Cproquest_cross%3E69713366%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c456t-f271d1e6ef66ebdef71d81571b0a008140a3aff3587395461a3cbbe045d1754f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=69713366&rft_id=info:pmid/10218796&rfr_iscdi=true |