Liquid chromatographic–electrospray tandem mass spectrometric determination of novel antifungal agents in plasma

A rapid, selective, sensitive and reproducible HPLC–electrospray tandem mass spectrometric method has been developed for the analysis of novel triazole antifungal agents, SYN-2869 and its derivatives (SYN-2836, SYN-2903 and SYN-2921), in rat plasma using SYN-2506 as an internal standard. Isolation o...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1999-03, Vol.724 (2), p.311-317
Main Authors: Bu, Hai-Zhi, Khan, Jehangir K., Poglod, Marzena, Micetich, Ronald G.
Format: Article
Language:English
Subjects:
Analysis
Animals
Antifungal agents
Antifungal Agents - blood
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Piperazines - blood
Rats
Reproducibility of Results
Sensitivity and Specificity
SYN-2869
Triazoles - blood
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Summary:A rapid, selective, sensitive and reproducible HPLC–electrospray tandem mass spectrometric method has been developed for the analysis of novel triazole antifungal agents, SYN-2869 and its derivatives (SYN-2836, SYN-2903 and SYN-2921), in rat plasma using SYN-2506 as an internal standard. Isolation of these compounds from plasma and sample desalting were performed by a simple extraction procedure involving protein precipitation, vacuum-drying and reconstitution with acetonitrile. For all the agents, linearity was observed over the range of 10–10 000 ng/ml ( r≥0.996) and the limit of quantitation was 10 ng/ml using a 100-μl plasma volume. A measurement rate of 400–500 samples/day/instrument could be achieved using this method.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(99)00002-X