The kidney expression of matrix metalloproteinase-9 in the diabetic nephropathy of Kkay mice

Abstract Background Disorder of the extracellular matrix has been suggested to play a role in the development of diabetic nephropathy. The aim of the present study was to examine the expression of matrix metalloproteinase-9 (MMP-9) in the kidney of Kkay mice, a type 2 diabetic model. Methods Eight K...

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Published in:Journal of diabetes and its complications 2008-11, Vol.22 (6), p.408-412
Main Authors: Qing-Hua, Guo, Ju-Ming, Lu, Chang-Yu, Pan, Zhao-Hui, Lu, Xiao-Man, Zou, Yi-Ming, Mu
Format: Article
Language:English
Subjects:
Animals
Blood Glucose - analysis
Diabetes
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - enzymology
Diabetic Nephropathies - enzymology
Diabetic Nephropathies - pathology
Diabetic nephropathy
Endocrinology & Metabolism
Gene Expression
Immunohistochemistry
Kidney - chemistry
Kidney - metabolism
Kidney - pathology
Kidney Glomerulus - enzymology
Kidney Glomerulus - pathology
Kidneys
Kkay mice
Matrix Metalloproteinase 9 - analysis
Matrix Metalloproteinase 9 - genetics
Matrix metalloproteinase-9
Mice
Proteins
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Rodents
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Summary:Abstract Background Disorder of the extracellular matrix has been suggested to play a role in the development of diabetic nephropathy. The aim of the present study was to examine the expression of matrix metalloproteinase-9 (MMP-9) in the kidney of Kkay mice, a type 2 diabetic model. Methods Eight Kkay control mice (KA) and eight Kkay diabetic mice (KB) were fed with the same diets for 4 months and sacrificed after glucose levels and weight were measured. The renal pathological changes were examined by periodic acid–Schiff and periodic acid–silver methenamine staining, and the glomerulosclerosis indexes (GIs) were calculated. The levels of MMP-9 protein in the kidney tissue were assessed by immunohistochemistry. The gene expression of MMP-9 was measured by reverse transcription polymerase chain reaction. Results The diabetic group had morphological findings of diabetic nephropathy, and their GI (240.0±17.3) was much higher than that of the control group (118.4±15.1) ( P
ISSN:1056-8727
1873-460X
DOI:10.1016/j.jdiacomp.2007.10.002