Characterization of Anti-heart Antibodies in Mice after Infection with Coxsackie B3 Virus

Coxsackie virus B3 (CVB3) infection results in a marked inflammatory response and the production of autoantibodies to cardiac antigens, with cardiac myosin heavy chain documented to be the most immunogenic antigen. The present study investigated the temporal appearance of anti-heart antibodies in mi...

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Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 1999-04, Vol.91 (1), p.90-98
Main Authors: Latif, Najma, Zhang, Hongyi, Archard, Leonard C., Yacoub, Magdi H., Dunn, Michael J.
Format: Article
Language:English
Subjects:
Actins - immunology
Animals
autoantibodies
Autoantibodies - biosynthesis
Autoantigens - isolation & purification
autoimmunity
biochemistry
Biological and medical sciences
Coxsackievirus Infections - etiology
Coxsackievirus Infections - immunology
Coxsackievirus Infections - pathology
Enterovirus B, Human - pathogenicity
Experimental and animal immunopathology. Animal models
Human coxsackievirus B3
Human viral diseases
Immunoglobulin G - biosynthesis
Immunopathology
Infectious diseases
infectious immunity virus
Male
Medical sciences
Mice
Myocarditis - etiology
Myocarditis - immunology
Myocarditis - pathology
Myocardium - immunology
Myosin Heavy Chains - immunology
Time Factors
Tropomyosin - immunology
Viral cardiopathies
Viral diseases
Virulence
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Summary:Coxsackie virus B3 (CVB3) infection results in a marked inflammatory response and the production of autoantibodies to cardiac antigens, with cardiac myosin heavy chain documented to be the most immunogenic antigen. The present study investigated the temporal appearance of anti-heart antibodies in mice after mock infection or infection with an attenuated variant of CVB3 or wildtpye CVB3 by SDS–PAGE and Western blotting. Further characterization of the autoantigens was carried out using 2D electrophoresis followed by Western blotting. Mice infected with wildtype CVB3 demonstrated high levels of IgG anti-heart antibodies, reacting predominantly with myosin heavy chain but also with numerous other myocardial proteins. Significant increases in anti-myosin heavy chain, anti-actin, and anti-tropomyosin antibodies were seen in wildtype-infected mice as early as day 7 postinfection compared to those mice that were mock infected or infected with attenuated virus. Characterization of other antigens revealed novel reactivities against myosin subfragments, heat shock proteins, and desmin and its subfragments.
ISSN:1521-6616
1521-7035
DOI:10.1006/clim.1998.4679