Lateral gene transfer of O1 serogroup encoding genes of Vibrio cholerae

In Gram-negative bacteria, the O-antigen-encoding genes may be transferred between lineages, although mechanisms are not fully understood. To assess possible lateral gene transfer (LGT), 21 Argentinean Vibrio cholerae O-group 1 (O1) isolates were examined using multilocus sequence typing (MLST) to d...

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Bibliographic Details
Published in:FEMS microbiology letters 2008-09, Vol.286 (1), p.32-38
Main Authors: Fraga, Sol González, Pichel, Mariana, Binsztein, Norma, Johnson, Judith A, Morris, John Glenn Jr, Colin Stine, Oscar
Format: Article
Language:English
Subjects:
Antigens
Bacteria
Bacterial Proteins - genetics
Base Sequence
Cholera - microbiology
Conserved sequence
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Gene transfer
Gene Transfer, Horizontal
Genes
Genetic Linkage
Gram-negative bacteria
Humans
lateral gene transfer
Loci
Microbiology
Molecular Sequence Data
Multigene Family
Multilocus sequence typing
Nucleotide sequence
Nucleotides
O Antigens - genetics
Pathogens
Sequence Alignment
Vibrio cholerae
Vibrio cholerae - classification
Vibrio cholerae - genetics
Vibrio cholerae - isolation & purification
Waterborne diseases
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Summary:In Gram-negative bacteria, the O-antigen-encoding genes may be transferred between lineages, although mechanisms are not fully understood. To assess possible lateral gene transfer (LGT), 21 Argentinean Vibrio cholerae O-group 1 (O1) isolates were examined using multilocus sequence typing (MLST) to determine the genetic relatedness of housekeeping genes and genes from the O1 gene cluster. MSLT analysis revealed that 4.4% of the nucleotides in the seven housekeeping loci were variable, with six distinct genetic lineages identified among O1 isolates. In contrast, MLST analysis of the eight loci from the O1 serogroup region revealed that 0.24% of the 4943 nucleotides were variable. A putative breakpoint was identified in the JUMPstart sequence. Nine conserved nucleotides differed by a single nucleotide from a DNA uptake signal sequence (USS) also found in Pastuerellaceae. Our data indicate that genes in the O1 biogenesis region are closely related even in distinct genetic lineages, indicative of LGT, with a putative DNA USS identified at the defined boundary for the DNA exchange.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2008.01251.x