Apolipoprotein E (ApoE), a Bmp-2 (bone morphogenetic protein) upregulated gene in mesenchymal progenitors (C3H10T1/2), is highly expressed in murine embryonic development

Apolipoprotein E (ApoE) was identified as upregulated by Bmp‐2 (bone morphogenetic protein‐2) in the murine mesenchymal progenitor cell line C3H10T1/2 by a subtractive cloning strategy. Expression of recombinant Bmps in mesenchymal C3H10T1/2 progenitors results in the differentiation into the osteog...

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Bibliographic Details
Published in:BioFactors (Oxford) 1999, Vol.9 (1), p.11-17
Main Authors: Bächner, Dietmar, Schröder, Dietmar, Betat, Nicole, Ahrens, Marion, Gross, Gerhard
Format: Article
Language:English
Subjects:
Animals
ApoE
Apolipoproteins E - genetics
Apolipoproteins E - metabolism
Bmp
Bone Morphogenetic Proteins - genetics
Bone Morphogenetic Proteins - physiology
C3H10T1/2
Cell Differentiation
Cell Line
Cell Lineage
Cells, Cultured
Cloning, Molecular
Embryo, Mammalian - cytology
Embryo, Mammalian - metabolism
Embryonic and Fetal Development
Gene Expression Regulation, Developmental
In Situ Hybridization
mesenchymal differentiation
Mesoderm - cytology
Mesoderm - metabolism
Mice
Mice, Inbred Strains
neural crest
Neural Crest - cytology
Neural Crest - embryology
Neural Crest - metabolism
Osteoblasts - cytology
Osteoblasts - metabolism
Recombinant Proteins - metabolism
Stem Cells - metabolism
subtractive cloning
Transfection
Up-Regulation
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Summary:Apolipoprotein E (ApoE) was identified as upregulated by Bmp‐2 (bone morphogenetic protein‐2) in the murine mesenchymal progenitor cell line C3H10T1/2 by a subtractive cloning strategy. Expression of recombinant Bmps in mesenchymal C3H10T1/2 progenitors results in the differentiation into the osteogenic, the chondrogenic, and the adipogenic lineage. In addition, ApoE is also expressed in primary osteoblasts isolated from murine calvariae late in the in vitro osteoblast developmental sequence. To infer possible roles of ApoE in organogenesis and tissue differentiation, ApoE expression during mouse embryonic development was analyzed in murine midgestation and late embryonic development by in situ hybridization. ApoE is highly expressed at many sites of organ development (liver, brain, heart, eye, lung), probably in a subset of neural crest cells and ectodermal derivatives suggestive for important functions of ApoE during embryonic differentiation and organ development.
ISSN:0951-6433
1872-8081
DOI:10.1002/biof.5520090103