Determination of Cyclosporin A in 20% Ethanol by a Magnetic Beads-Based Immunofluorescence Assay

A rapid magnetic beads-based immunoassay for the immunodepressant drug cyclosporin A (CsA) has been developed. The method allows CsA determination in medium with a higher content of ethanol compared to conventional immunochemical techniques due to increased antibody stability. Monitoring of the drug...

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Bibliographic Details
Published in:Analytical biochemistry 1999-05, Vol.269 (2), p.393-398
Main Authors: Kiselev, Maxim V., Gladilin, Alexander K., Melik-Nubarov, Nikolay S., Sveshnikov, Peter G., Miethe, Peter, Levashov, Andrey V.
Format: Article
Language:English
Subjects:
Antibody Affinity
Blood Chemical Analysis - methods
Blood Chemical Analysis - statistics & numerical data
Cyclosporine - blood
Cyclosporine - immunology
Ethanol
Fluorescent Antibody Technique - statistics & numerical data
Humans
Immunosuppressive Agents - blood
Immunosuppressive Agents - immunology
Magnetics
Sensitivity and Specificity
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Summary:A rapid magnetic beads-based immunoassay for the immunodepressant drug cyclosporin A (CsA) has been developed. The method allows CsA determination in medium with a higher content of ethanol compared to conventional immunochemical techniques due to increased antibody stability. Monitoring of the drug in ethanol extracts from patient's whole blood without many-fold dilution with aqueous buffer is possible. The assay has adequate specificity and sensitivity for CsA to be suitable for the routine monitoring of therapy.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1999.4015