Characterization of an alphoid subfamily located near p-arm sequences on human chromosome 22

The centromeric heterochromatin of all human chromosomes is composed of tandemly repeated alpha satellite DNA. Here we describe another alphoid subfamily that maps to human chromosome 22 as determined by FISH. The alphoid sequences were isolated from three YAC-clones carrying DNA from the pericentro...

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Bibliographic Details
Published in:Chromosome research 1999-01, Vol.7 (1), p.65-69
Main Authors: Eisenbarth, I, König-Greger, D, Wöhr, G, Kehrer-Sawatzki, H, Assum, G
Format: Article
Language:English
Subjects:
Blotting, Southern
Centromere
Chromosomes
Chromosomes, Artificial, Yeast - genetics
Chromosomes, Human, Pair 22
Deoxyribonucleic acid
DNA
DNA Probes
DNA, Satellite
Genetics
Humans
In Situ Hybridization, Fluorescence
Male
Polymerase Chain Reaction
Restriction Mapping
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Summary:The centromeric heterochromatin of all human chromosomes is composed of tandemly repeated alpha satellite DNA. Here we describe another alphoid subfamily that maps to human chromosome 22 as determined by FISH. The alphoid sequences were isolated from three YAC-clones carrying DNA from the pericentromeric region of the short arm of human chromosome 22 and limited amounts of alphoid DNA. This property enabled us to map the members of the subfamily to the border of the centromeric region and the short arm of the chromosome. The new alphoid subfamily may contribute to the closure of the gap remaining between the centromeric and short-arm maps of human chromosome 22.
ISSN:0967-3849
1573-6849
DOI:10.1023/A:1009279412797