Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously...

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Bibliographic Details
Published in:Veterinary microbiology 1999-04, Vol.66 (2), p.115-123
Main Authors: Allan, G.M, Mc Neilly, F, Meehan, B.M, Kennedy, S, Mackie, D.P, Ellis, J.A, Clark, E.G, Espuna, E, Saubi, N, Riera, P, Bøtner, A, Charreyre, C.E
Format: Article
Language:English
Subjects:
Animal viral diseases
Animals
ANTICORPS MONOCLONAL
ANTICORPS POLYCLONAL
ANTICUERPOS MONOCLONALES
ANTICUERPOS POLICLONALES
ANTIGENE
ANTIGENOS
ANTIGENS
Biological and medical sciences
CERDO
CIRCOVIRIDAE
Circoviridae Infections
Circoviridae Infections - physiopathology
Circoviridae Infections - veterinary
Circovirus
Circovirus - classification
Circovirus - genetics
Circovirus - isolation & purification
classification
DANEMARK
DENMARK
DINAMARCA
DNA Primers
Epidemiology
ESPAGNE
ESPANA
Fundamental and applied biological sciences. Psychology
genetics
GENOTIPOS
GENOTYPE
GENOTYPES
Genotyping
Infectious diseases
IRLANDA DEL NORTE
IRLANDE DU NORD
isolation & purification
Medical sciences
Microbiology
MONOCLONAL ANTIBODIES
NORTHERN IRELAND
NUTRITIONAL DISORDERS
PCR
physiopathology
POLYCLONAL ANTIBODIES
Polymerase Chain Reaction
PORCIN
Porcine circovirus
SPAIN
strains
SWINE
Swine Diseases
Swine Diseases - physiopathology
Swine Diseases - virology
TRASTORNOS NUTRICIONALES
TROUBLE NUTRITIONNEL
veterinary
Viral diseases
Virology
Wasting Syndrome
Wasting Syndrome - physiopathology
Wasting Syndrome - veterinary
Wasting Syndrome - virology
Wasting syndromes
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Description
Summary:A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(99)00004-8