Overexpression of c-Src Enhances Cell-Matrix Adhesion and Cell Migration in PDGF-Stimulated NIH3T3 Fibroblasts

c-Src is normally associated with the plasma membrane, but upon activation by tyrosine kinase receptors it translocates to the cytoskeleton. Activation of c-Src alters its conformation and induces the association of c-Src with cytoskeletal proteins. c-Src is implicated in tyrosine phosphorylation of...

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Bibliographic Details
Published in:Experimental cell research 1999-05, Vol.248 (2), p.531-537
Main Authors: Verbeek, Bianca S., Vroom, Thea M., Rijksen, Gert
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Biological Transport
c-Src
Cell Adhesion
Cell Compartmentation
cell migration
Cell Movement
Cell Transformation, Neoplastic
Cytoskeleton
Extracellular Matrix
Mice
overexpression
PDGF receptor
Platelet-Derived Growth Factor - pharmacology
Proto-Oncogene Proteins pp60(c-src) - biosynthesis
Proto-Oncogene Proteins pp60(c-src) - genetics
Recombinant Proteins - biosynthesis
Space life sciences
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Summary:c-Src is normally associated with the plasma membrane, but upon activation by tyrosine kinase receptors it translocates to the cytoskeleton. Activation of c-Src alters its conformation and induces the association of c-Src with cytoskeletal proteins. c-Src is implicated in tyrosine phosphorylation of cytoskeletal proteins, which might affect the cytoskeletal architecture. Rearrangements of the cytoskeleton affect cell-matrix adhesion and cell migration. In this study NIH3T3 fibroblasts, that overexpress c-Src, were used to analyze the effect of c-Src on both cell-matrix adhesion and cell migration. Upon PDGF stimulation translocation of c-Src to the cytoskeleton was detected. PDGF treatment also increased cell-matrix adhesion and cell migration. The cell line with the highest c-Src expression showed the largest increases in both phenomena. These findings suggest that translocation of c-Src to the cytoskeleton results in enhanced cell-matrix adhesion and cell migration.
ISSN:0014-4827
1090-2422
DOI:10.1006/excr.1999.4416