Evidence of LMP1–TRAF3 interactions in glycosphingolipid‐rich complexes of lymphoblastoid and nasopharyngeal carcinoma cells

Latent membrane protein 1 (LMP1) is an Epstein‐Barr virus (EBV) protein expressed in EBV‐transformed B lymphocytes and in approximately 50% of nasopharyngeal carcinomas (NPCs). LMP1 signaling involves several cellular signaling intermediates, especially TNF receptor–associated factors (TRAFs). We ha...

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Bibliographic Details
Published in:International journal of cancer 1999-05, Vol.81 (4), p.645-649
Main Authors: Ardila‐Osorio, Hector, Clausse, Bernard, Mishal, Zohair, Wiels, Joëlle, Tursz, Thomas, Busson, Pierre
Format: Article
Language:English
Subjects:
Animals
Biopsy
Burkitt Lymphoma - chemistry
Burkitt Lymphoma - metabolism
Burkitt Lymphoma - pathology
Centrifugation, Density Gradient
Glycosphingolipids - isolation & purification
Glycosphingolipids - metabolism
Herpesvirus 4, Human - genetics
Humans
Mice
Mice, Nude
Nasopharyngeal Neoplasms - chemistry
Nasopharyngeal Neoplasms - metabolism
Nasopharyngeal Neoplasms - pathology
Proteins - genetics
Proteins - isolation & purification
Proteins - metabolism
Receptors, Tumor Necrosis Factor - physiology
TNF Receptor-Associated Factor 3
Transplantation, Heterologous
Tumor Cells, Cultured
Viral Matrix Proteins - genetics
Viral Matrix Proteins - isolation & purification
Viral Matrix Proteins - metabolism
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Summary:Latent membrane protein 1 (LMP1) is an Epstein‐Barr virus (EBV) protein expressed in EBV‐transformed B lymphocytes and in approximately 50% of nasopharyngeal carcinomas (NPCs). LMP1 signaling involves several cellular signaling intermediates, especially TNF receptor–associated factors (TRAFs). We have shown previously that LMP1 is highly concentrated in a cell fraction called glycosphingolipid‐rich membrane complexes (GSL complexes). We report here that parallel accumulation of LMP1 and TRAF3, but not TRAF1 or TRADD, was observed in GSL complexes from lymphoblastoid and LMP1‐positive NPC cells. In contrast, TRAF3 was not concentrated in GSL complexes from LMP1‐negative cells. Binding of LMP1 and TRAF3 in GSL complexes was demonstrated in lymphoblastoid and NPC cells, by co‐immunoprecipitation with both anti‐LMP1 and anti‐TRAF3 antibodies. Int. J. Cancer 81:645–649, 1999. © 1999 Wiley‐Liss, Inc.
ISSN:0020-7136
1097-0215
DOI:10.1002/(SICI)1097-0215(19990517)81:4<645::AID-IJC22>3.0.CO;2-0