Modulation of hepatic and intestinal Glutathione S-transferases and other antioxidant enzymes by dietary lipids in streptozotocin diabetic rats

• Published in: Chemosphere (Oxford) 1999-06, Vol.38 (13), p.3003-3013, Article 3003
• Main Authors: Girón, M.Dolores, Salto, Rafael, González, Yolanda, Girón, Jose A., Nieto, Natalia, Periago, JoséL., Suàrez, M.Dolores, Hortelano, Paloma
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant defenses; Antioxidants - metabolism; Associated diseases and complications; Biological and medical sciences; Blood Glucose - metabolism; Blotting, Western; Diabetes Mellitus, Experimental - enzymology; Diabetes. Impaired glucose tolerance; Dietary fat; Dietary Fats - pharmacology; Dietary Fats, Unsaturated - pharmacology; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Fatty Acids - pharmacology; Female; Fish Oils - pharmacology; Glutathione Peroxidase - metabolism; Glutathione Transferase - metabolism; Intestines - drug effects; Intestines - enzymology; Isoenzymes - metabolism; Liver - drug effects; Liver - enzymology; Medical sciences; Olive Oil; Plant Oils - pharmacology; Rats; Rats, Wistar; Streptozotocin diabetic rats; Sunflower Oil; Superoxide Dismutase - metabolism
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/S0045-6535(98)00502-5

Antioxidant enzymes in liver and small intestine were investigated using control and streptozotocin diabetic rats fed diets with 5% olive, sunflower or fish oil for five weeks. In liver, Glutathione Peroxidase and Superoxide Dismutase decreased and in intestine Glutathione-S-transferase (GST) increased by diabetes. In isolated jejunum and ileum, this increase in GST activity was due to an increase in GST-α and -μ isoenzymes in jejunum and GST-α, μ and -π in ileum. Since GST plays an important role in protecting tissues from oxidative damage, our results highlight the role of the intestine against free radicals in physiological or pathological situations.