Bacterial Peptidoglycan Polysaccharides in Sterile Human Spleen Induce Proinflammatory Cytokine Production by Human Blood Cells

Peptidoglycan (PG) is the major component of the cell wall of gram-positive bacteria. In vitro, PG isolated from conventional bacterial cultures can induce secretion of proinflammatory cytokines by human monocytes, indicating that PG may be involved in immune responses against infections by gram-pos...

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Bibliographic Details
Published in:The Journal of infectious diseases 1999-06, Vol.179 (6), p.1459-1468
Main Authors: Schrijver, Ingrid A., Melief, Marie-José, Eulderink, Frits, Hazenberg, Maarten P., Laman, Jon D.
Format: Article
Language:English
Subjects:
Antibodies
Antigen presenting cells
Bacteriology
Biological and medical sciences
Blood
Blood cells
Blood Cells - immunology
Cell walls
Cytokines
Cytokines - biosynthesis
Feces
Fundamental and applied biological sciences. Psychology
Gels
Gram-Positive Bacteria - immunology
Humans
In Vitro Techniques
Inflammation Mediators - metabolism
Interleukin-1 - biosynthesis
Interleukin-6 - biosynthesis
Major Articles
Microbiology
Monocytes
Muramic Acids - analysis
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Peptidoglycan
Peptidoglycan - immunology
Peptidoglycan - pharmacology
Spleen
Spleen - cytology
Spleen - drug effects
Spleen - immunology
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:Peptidoglycan (PG) is the major component of the cell wall of gram-positive bacteria. In vitro, PG isolated from conventional bacterial cultures can induce secretion of proinflammatory cytokines by human monocytes, indicating that PG may be involved in immune responses against infections by gram-positive bacteria. To investigate the biologic activity of PG in human tissues, an improved method was developed to isolate significant amounts of PG from sterile human spleen tissue. Biochemical analysis demonstrated that PG isolated from human spleen is largely intact. Human whole blood cell cultures were able to produce the proinflammatory cytokines tumor necrosis factor-α and interleukin-1 and -6 after stimulation with PG isolated from human spleen. Cytokine induction was not sensitive to inhibition by polymyxin B, in contrast to lipopolysaccharide. Collectively, the data show that intact PG in sterile human tissue is biologically active and may induce local proinflammatory cytokine production.
ISSN:0022-1899
1537-6613
DOI:10.1086/314761