Improving Specific Activity of Aspergillus carbonarius Polygalacturonase Using Polymeric Membranes

Microfiltration (MF) and ultrafiltration (UF) membranes were screened for improving the specific activity of polygalacturonases (PG) in the culture broth of Aspergillus carbonarius obtained after submerged fermentation. While 200 and 450 nm MF membranes eliminated some of the larger non-enzymatic pr...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2008-12, Vol.151 (2-3), p.233-243
Main Authors: Nakkeeran, E, Subramanian, R, Umesh Kumar, S
Format: Article
Language:English
Subjects:
artificial membranes
Aspergillus - enzymology
Aspergillus carbonarius
Biochemistry
Biotechnology
Chemistry
Chemistry and Materials Science
culture media
enzyme activity
Enzymes
Fermentation
Membrane processes
Membranes
Membranes, Artificial
microfiltration
polygalacturonase
Polygalacturonase - isolation & purification
Polygalacturonase - metabolism
Polymers
Proteins
submerged fermentation
Ultrafiltration
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Summary:Microfiltration (MF) and ultrafiltration (UF) membranes were screened for improving the specific activity of polygalacturonases (PG) in the culture broth of Aspergillus carbonarius obtained after submerged fermentation. While 200 and 450 nm MF membranes eliminated some of the larger non-enzymatic proteins, 50 kDa UF membrane exhibited a marginal selectivity between the enzyme and other smaller proteins. The 450 nm MF and 50 kDa UF membranes selected were further evaluated under different process conditions for an integrated membrane process. The process efficacy of three different schemes was also studied for enzyme purification. A two-stage membrane process employing MF followed by UF improved the enzyme-specific activity (5,590 U/mg) by 4.69-fold eliminating the larger and smaller non-enzymatic proteins as well as non-protein impurities with a recovery of 76% enzymes, besides resulting in higher productivity. Thus, adoption of integrated membrane process with appropriate selection of membranes could result in high recovery of enzymes with improved specific activity.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-008-8182-7