Induction of experimental autoimmune thyroiditis by heat‐denatured porcine thyroglobulin: a Tc1‐mediated disease

• Published in: European journal of immunology 1999-04, Vol.29 (4), p.1342-1352
• Main Authors: Brazillet, Marie‐Pierre, Batteux, Frédéric, Abehsira‐Amar, Orly, Nicoletti, Ferdinando, Charreire, Jeannine
• Format: Article
• Language: English
• Subjects: Animals; Autoimmunity; Cytotoxic T lymphocyte; Cytotoxicity, Immunologic; Disease Models, Animal; Female; Histocompatibility Antigens Class I - physiology; Hot Temperature; Immunoglobulin Isotypes - blood; In vivo animal model; Interferon-gamma - genetics; Lymphocyte Activation; Mice; Mice, Inbred CBA; Protein Denaturation; RNA, Messenger - analysis; Swine; T-Lymphocytes, Cytotoxic - immunology; Tc1; Thyroglobulin - immunology; Thyroiditis, Autoimmune - etiology; Thyroiditis, Autoimmune - pathology
• ISSN: 0014-2980; 1521-4141
• DOI: 10.1002/(SICI)1521-4141(199904)29:04<1342::AID-IMMU1342>3.0.CO;2-A

Recent studies have shown that denatured exogenous antigens can prime cytotoxic T lymphocytes (CTL). To assess the contribution of CTL to experimental autoimmune thyroiditis (EAT), porcine thyroglobulin (pTg) was heat‐denatured (hdpTg) and injected i.  v. into CBA/J mice, without the aid of adjuvants. Both lymphocytic infiltrations of the thyroid glands and levels of Tg‐specific CTL were similar to those found in conventional EAT induced by Tg and adjuvants. In contrast, proliferative responses could not be detected, and titers of antibodies to pTg were 20 times lower. These EAT‐inducer CTL belong to the CD8+ cell subset and exerted their thyroiditogenic potential through release of IFN‐γ. We conclude that hdpTg‐induced EAT is mediated by type 1 cytotoxic T cells (Tc1).