Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant

• Published in: Theoretical and applied genetics 2008-11, Vol.117 (8), p.1303-1312
• Main Authors: Mutlu, Nedim, Boyacı, Filiz Hatice, Göçmen, Münevver, Abak, Kazım
• Format: Article
• Language: English
• Subjects: Agricultural research; Agriculture; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Chromosomes, Plant; Classical genetics, quantitative genetics, hybrids; Disease; DNA Primers; DNA, Plant - genetics; Fundamental and applied biological sciences. Psychology; Fusarium - pathogenicity; Genes; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; Greenhouses; Immunity, Innate; Life Sciences; Original Paper; Pathogens; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Genetics and Genomics; Plant resistance; Polymorphism; Pteridophyta, spermatophyta; Random Amplified Polymorphic DNA Technique; Research centers; Sequence Analysis, DNA; Solanum melongena - genetics; Solanum melongena - microbiology; Vegetals
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-008-0864-6

Fusarium wilt (Fusarium oxysporum Schlecht. f. sp. melongenae) is a vascular disease of eggplant (Solanum melongena L.). The objectives of this work were (1) to confirm the monogenic inheritance of fusarium wilt resistance in eggplant, (2) to identify molecular markers linked to this resistance, and (3) to develop SCAR markers from most informative markers. We report the tagging of the gene for resistance to fusarium wilt (FOM) in eggplant using SRAP, RGA, SRAP-RGA and RAPD markers. Analysis of segregation data confirmed the monogenic inheritance of resistance. DNA from F₂ and BC₁ populations of eggplant segregating for fusarium wilt resistance was screened with 2,316 primer combinations to detect polymorphism. Three markers were linked within 2.6 cM of the gene. The codominant SRAP marker Me8/Em5 and dominant SRAP-RGA marker Em12/GLPL2 were tightly linked to each other and mapped 1.2 cM from the resistance gene, whereas RAPD marker H12 mapped 2.6 cM from the gene and on the same side as the other two markers. The SRAP marker was converted into two dominant SCAR markers that were confirmed to be linked to the resistance gene in the F₂, BC₁ and F₂ of BC₃ generations of the same cross. These markers provide a starting point for mapping the eggplant FOM resistance gene in eggplant and for exploring the synteny between solanaceous crops for fusarium wilt resistance genes. The SCAR markers will be useful for identifying fusarium wilt-resistant genotypes in marker-assisted selection breeding programs using segregating progenies of the resistant eggplant progenitor used in this study.