Augmentation of the CD8+ T cell response by IFN-gamma in IL-12-deficient mice during Toxoplasma gondii infection

The importance of IFN-gamma in regulating the host CD8+ T cell response during microbial infection has not been delineated. Mice deficient for the p40 chain of the IL-12 heterodimer have impaired IFN-gamma production and are susceptible to infection with the intracellular parasite Toxoplasma gondii....

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 1999-05, Vol.162 (9), p.5449-5454
Main Authors: Ely, K H, Kasper, L H, Khan, I A
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - physiology
Adoptive Transfer
AIDS/HIV
Animals
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - metabolism
CD8-Positive T-Lymphocytes - transplantation
Disease Susceptibility
Down-Regulation
Female
Interferon-gamma - antagonists & inhibitors
Interferon-gamma - biosynthesis
Interferon-gamma - physiology
Interleukin-12 - deficiency
Interleukin-12 - genetics
Lymphocyte Count
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Stem Cells - immunology
Temperature
Time Factors
Toxoplasma - classification
Toxoplasma - genetics
Toxoplasma - immunology
Toxoplasmosis, Animal - genetics
Toxoplasmosis, Animal - immunology
Toxoplasmosis, Animal - mortality
Toxoplasmosis, Animal - prevention & control
Vaccination
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The importance of IFN-gamma in regulating the host CD8+ T cell response during microbial infection has not been delineated. Mice deficient for the p40 chain of the IL-12 heterodimer have impaired IFN-gamma production and are susceptible to infection with the intracellular parasite Toxoplasma gondii. The administration of exogenous IFN-gamma to parasite-infected p40-/- mice increases survival and up-regulates the depressed CD8+ T cell response following infection. CD8+ T cells isolated from cytokine-treated p40-/- mice exhibit an increase in both precursor CTL frequency and IFN-gamma production compared with untreated controls. The enhancement of the CD8+ T cell response is independent of CD4+ T cell help. These CD8+ T cells induce protective immunity against a lethal challenge when adoptively transferred into naive p40-/- and IFN-gamma-/- mice. These observations indicate that IFN-gamma can regulate the CD8+ T cell response during T. gondii infection.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.162.9.5449