Sulfated galactofucan from Lobophora variegata: Anticoagulant and anti-inflammatory properties

Sulfated polysaccharides (fucans and fucoidans) from brown algae show several biological activities, including anticoagulant and anti-inflammatory activities. We have extracted a sulfated heterofucan from the brown seaweed Lobophora variegata by proteolytic digestion, followed by acetone fractionati...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2008-09, Vol.73 (9), p.1018-1024
Main Authors: Medeiros, V. P., Queiroz, K. C. S., Cardoso, M. L., Monteiro, G. R. G., Oliveira, F. W., Chavante, S. F., Guimaraes, L. A., Rocha, H. A. O., Leite, E. L.
Format: Article
Language:English
Subjects:
Algae
Animals
Anti-Inflammatory Agents - chemistry
Anti-Inflammatory Agents - isolation & purification
Anti-Inflammatory Agents - pharmacology
Anticoagulants
Anticoagulants - chemistry
Anticoagulants - isolation & purification
Anticoagulants - pharmacology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Comparative analysis
Croton
Enzymes
Fractionation
Fucus vesiculosus
Galactans - chemistry
Galactans - isolation & purification
Galactans - pharmacology
Infrared spectroscopy
Life Sciences
Lobophora variegata
Microbiology
Phaeophyceae - chemistry
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Rats
Saccharides
Studies
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Summary:Sulfated polysaccharides (fucans and fucoidans) from brown algae show several biological activities, including anticoagulant and anti-inflammatory activities. We have extracted a sulfated heterofucan from the brown seaweed Lobophora variegata by proteolytic digestion, followed by acetone fractionation, molecular sieving, and ion-exchange chromatography. Chemical analyses and 13 C-NMR and IR spectroscopy showed that this fucoidan is composed of fucose, galactose, and sulfate at molar ratios of 1:3:2. We compared the anticoagulant activity of L. variegata fucoidan with those of a commercial sulfated polysaccharide (also named fucoidan) from Fucus vesiculosus and heparin. The experimental inflammation models utilized in this work revealed that fucoidan from L. variegata inhibits leukocyte migration to the inflammation site. Ear swelling caused by croton oil was also inhibited when sulfated polysaccharides from F. vesiculosus and L. variegata were used. The precise mechanism of different action between homo-and heterofucans is not clear; nevertheless, the polysaccharides studied here may have therapeutic potential in inflammatory disorders.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297908090095