Arachidonoyl‐CoA: Lysophosphatidylcholine acyltransferase activity in ras‐transformed NIH 3T3 fibroblasts depends on the membrane composition

• Published in: Biochemistry and molecular biology international 1999-04, Vol.47 (4), p.555-561
• Main Authors: Momchilova, Albena, Markovska, Tania, Pankov, Roumen
• Format: Article
• Language: English
• Subjects: 3T3 Cells; acyltransferase; Acyltransferases - metabolism; Animals; Cell Line, Transformed; ceramide; diacylglycerol; Humans; Intracellular Membranes - drug effects; Intracellular Membranes - metabolism; Membrane Lipids - metabolism; Mice; Microsomes - drug effects; Microsomes - metabolism; Phospholipids - metabolism; Phospholipids - pharmacology; ras; ras Proteins - genetics; ras Proteins - metabolism; sphingomyelin
• ISSN: 1521-6543; 1039-9712; 1521-6551
• DOI: 10.1080/15216549900201593

Investigations were performed on the influence of membrane lipids on arachidonoyl‐CoA: lysophosphatidylcholine acyltransferase in microsomal membranes from control and ras‐transformed NIH 3T3 fibroblasts. Of all the tested phospholipids only sphingomyelin induced activation of acyltransferase in membranes from ras‐transformed cells. No specific phospholipid effect on the acyltransferase was observed in microsomal membranes from control fibroblasts. Diacylglycerol was found to inhibit acyltransferase in both cell lines, whereas ceramide accumulation induced inhibition only in membranes from the transformed cells. The effects of diacylglycerol, ceramide, sphingomyelin and sphingomyelinase are discussed with respect to their putative roles in the signal transduction pathways in oncogene‐expressing cells.