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Arachidonoyl‐CoA: Lysophosphatidylcholine acyltransferase activity in ras‐transformed NIH 3T3 fibroblasts depends on the membrane composition
Investigations were performed on the influence of membrane lipids on arachidonoyl‐CoA: lysophosphatidylcholine acyltransferase in microsomal membranes from control and ras‐transformed NIH 3T3 fibroblasts. Of all the tested phospholipids only sphingomyelin induced activation of acyltransferase in mem...
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Published in: | Biochemistry and molecular biology international 1999-04, Vol.47 (4), p.555-561 |
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container_title | Biochemistry and molecular biology international |
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creator | Momchilova, Albena Markovska, Tania Pankov, Roumen |
description | Investigations were performed on the influence of membrane lipids on arachidonoyl‐CoA: lysophosphatidylcholine acyltransferase in microsomal membranes from control and ras‐transformed NIH 3T3 fibroblasts. Of all the tested phospholipids only sphingomyelin induced activation of acyltransferase in membranes from ras‐transformed cells. No specific phospholipid effect on the acyltransferase was observed in microsomal membranes from control fibroblasts. Diacylglycerol was found to inhibit acyltransferase in both cell lines, whereas ceramide accumulation induced inhibition only in membranes from the transformed cells. The effects of diacylglycerol, ceramide, sphingomyelin and sphingomyelinase are discussed with respect to their putative roles in the signal transduction pathways in oncogene‐expressing cells. |
doi_str_mv | 10.1080/15216549900201593 |
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The effects of diacylglycerol, ceramide, sphingomyelin and sphingomyelinase are discussed with respect to their putative roles in the signal transduction pathways in oncogene‐expressing cells.</description><identifier>ISSN: 1521-6543</identifier><identifier>ISSN: 1039-9712</identifier><identifier>EISSN: 1521-6551</identifier><identifier>DOI: 10.1080/15216549900201593</identifier><identifier>PMID: 10319406</identifier><language>eng</language><publisher>UK: Informa Healthcare</publisher><subject>3T3 Cells ; acyltransferase ; Acyltransferases - metabolism ; Animals ; Cell Line, Transformed ; ceramide ; diacylglycerol ; Humans ; Intracellular Membranes - drug effects ; Intracellular Membranes - metabolism ; Membrane Lipids - metabolism ; Mice ; Microsomes - drug effects ; Microsomes - metabolism ; Phospholipids - metabolism ; Phospholipids - pharmacology ; ras ; ras Proteins - genetics ; ras Proteins - metabolism ; sphingomyelin</subject><ispartof>Biochemistry and molecular biology international, 1999-04, Vol.47 (4), p.555-561</ispartof><rights>Copyright © 1999 International Union of Biochemistry and Molecular Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10319406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Momchilova, Albena</creatorcontrib><creatorcontrib>Markovska, Tania</creatorcontrib><creatorcontrib>Pankov, Roumen</creatorcontrib><title>Arachidonoyl‐CoA: Lysophosphatidylcholine acyltransferase activity in ras‐transformed NIH 3T3 fibroblasts depends on the membrane composition</title><title>Biochemistry and molecular biology international</title><addtitle>Biochem Mol Biol Int</addtitle><description>Investigations were performed on the influence of membrane lipids on arachidonoyl‐CoA: lysophosphatidylcholine acyltransferase in microsomal membranes from control and ras‐transformed NIH 3T3 fibroblasts. 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The effects of diacylglycerol, ceramide, sphingomyelin and sphingomyelinase are discussed with respect to their putative roles in the signal transduction pathways in oncogene‐expressing cells.</description><subject>3T3 Cells</subject><subject>acyltransferase</subject><subject>Acyltransferases - metabolism</subject><subject>Animals</subject><subject>Cell Line, Transformed</subject><subject>ceramide</subject><subject>diacylglycerol</subject><subject>Humans</subject><subject>Intracellular Membranes - drug effects</subject><subject>Intracellular Membranes - metabolism</subject><subject>Membrane Lipids - metabolism</subject><subject>Mice</subject><subject>Microsomes - drug effects</subject><subject>Microsomes - metabolism</subject><subject>Phospholipids - metabolism</subject><subject>Phospholipids - pharmacology</subject><subject>ras</subject><subject>ras Proteins - genetics</subject><subject>ras Proteins - metabolism</subject><subject>sphingomyelin</subject><issn>1521-6543</issn><issn>1039-9712</issn><issn>1521-6551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFUU1P3DAQtaqiLtD-gF4qn3pb8KztOEa9LCu-pAUu23PkxI7iyomDnQXlxk8of5FfgldZoUocOpZm7DfvPVkzCH0HcgIkJ6fAF5BxJiUhCwJc0k_ocIfNM87h8_ud0Rk6ivEPSSGI_IJmQChIRrJD9LIMqmqs9p0f3evz35VfnuH1GH3f-Ng3arB6dFXjne0MVtXohqC6WJug4u492Ec7jNh2OAFJPnV9aI3GdzfXmG4orm0ZfOlUHCLWpjedjth3eGgMbk1bJoXBlW97H-1gffcVHdTKRfNtX4_R78uLzep6vr6_ulkt1_OKMi52Wcmca0bzElitMmJkrUELKXMDmVFScM15AiljGZGM00UJvBZ5OqVU9Bj9nHz74B-2Jg5Fa2NlnEv_8dtYZFIkb8ITESZiFXyMwdRFH2yrwlgAKXZ7KD7sIWl-7M23ZZrFP4pp8InwayI8WWfG_zsWm_Pbc8EBBNCFoG-kp5fF</recordid><startdate>199904</startdate><enddate>199904</enddate><creator>Momchilova, Albena</creator><creator>Markovska, Tania</creator><creator>Pankov, Roumen</creator><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199904</creationdate><title>Arachidonoyl‐CoA: Lysophosphatidylcholine acyltransferase activity in ras‐transformed NIH 3T3 fibroblasts depends on the membrane composition</title><author>Momchilova, Albena ; Markovska, Tania ; Pankov, Roumen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3457-c34a985d438b14fa60e9fd1d7998e16ea975d55e9f3446094532b15f78787b9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>3T3 Cells</topic><topic>acyltransferase</topic><topic>Acyltransferases - metabolism</topic><topic>Animals</topic><topic>Cell Line, Transformed</topic><topic>ceramide</topic><topic>diacylglycerol</topic><topic>Humans</topic><topic>Intracellular Membranes - drug effects</topic><topic>Intracellular Membranes - metabolism</topic><topic>Membrane Lipids - metabolism</topic><topic>Mice</topic><topic>Microsomes - drug effects</topic><topic>Microsomes - metabolism</topic><topic>Phospholipids - metabolism</topic><topic>Phospholipids - pharmacology</topic><topic>ras</topic><topic>ras Proteins - genetics</topic><topic>ras Proteins - metabolism</topic><topic>sphingomyelin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Momchilova, Albena</creatorcontrib><creatorcontrib>Markovska, Tania</creatorcontrib><creatorcontrib>Pankov, Roumen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry and molecular biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Momchilova, Albena</au><au>Markovska, Tania</au><au>Pankov, Roumen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arachidonoyl‐CoA: Lysophosphatidylcholine acyltransferase activity in ras‐transformed NIH 3T3 fibroblasts depends on the membrane composition</atitle><jtitle>Biochemistry and molecular biology international</jtitle><addtitle>Biochem Mol Biol Int</addtitle><date>1999-04</date><risdate>1999</risdate><volume>47</volume><issue>4</issue><spage>555</spage><epage>561</epage><pages>555-561</pages><issn>1521-6543</issn><issn>1039-9712</issn><eissn>1521-6551</eissn><abstract>Investigations were performed on the influence of membrane lipids on arachidonoyl‐CoA: lysophosphatidylcholine acyltransferase in microsomal membranes from control and ras‐transformed NIH 3T3 fibroblasts. 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subjects | 3T3 Cells acyltransferase Acyltransferases - metabolism Animals Cell Line, Transformed ceramide diacylglycerol Humans Intracellular Membranes - drug effects Intracellular Membranes - metabolism Membrane Lipids - metabolism Mice Microsomes - drug effects Microsomes - metabolism Phospholipids - metabolism Phospholipids - pharmacology ras ras Proteins - genetics ras Proteins - metabolism sphingomyelin |
title | Arachidonoyl‐CoA: Lysophosphatidylcholine acyltransferase activity in ras‐transformed NIH 3T3 fibroblasts depends on the membrane composition |
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