Establishment and characterization of a human neuroectodermal cell line (TB) from a cerebrospinal fluid specimen

We have established a cell line (TB) from a cerebrospinal fluid (CSF) specimen of a patient with a primary leptomeningeal melanomatosis. TB cell line was immunoreactive with the antibodies for low molecular weight neurofilament protein, vimentin, neuron-specific enolase, chromogranin, synaptophysin...

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Bibliographic Details
Published in:Brain research 1999-05, Vol.827 (1), p.205-209
Main Authors: Sorrentino, Giuseppe, Monsurrò, Maria R, Pettinato, Guido, Vanni, Roberta, Zuddas, Alessandro, Di Porzio, Umberto, Bonavita, Vincenzo
Format: Article
Language:English
Subjects:
Animal cells
Antibodies, Monoclonal
Antineoplastic Agents - pharmacology
Biological and medical sciences
Blotting, Western
Cell cultures. Hybridization. Fusion
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cerebrospinal Fluid - cytology
Chromogranins - analysis
Chromogranins - immunology
Fundamental and applied biological sciences. Psychology
Humans
Leptomeningeal melanoma
Meningeal Neoplasms
Molecular and cellular biology
Neural crest
Neural Crest - cytology
Neuroectodermal Tumor, Melanotic
Neuroendocrine differentiation
Neurofilament
Neurofilament Proteins - analysis
Neurofilament Proteins - immunology
Phosphopyruvate Hydratase - analysis
Phosphopyruvate Hydratase - immunology
Retinoic acid
Synaptophysin - analysis
Synaptophysin - immunology
Tretinoin - pharmacology
Tumor Cells, Cultured - chemistry
Tumor Cells, Cultured - cytology
Tumor Cells, Cultured - enzymology
Vimentin - analysis
Vimentin - immunology
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Summary:We have established a cell line (TB) from a cerebrospinal fluid (CSF) specimen of a patient with a primary leptomeningeal melanomatosis. TB cell line was immunoreactive with the antibodies for low molecular weight neurofilament protein, vimentin, neuron-specific enolase, chromogranin, synaptophysin and HMB-45 (an antibody sensitive and specific for melanoma). When TB cells were transplanted into nude mice, the same immunohistochemical pattern present in cultured cells was found but surprisingly, a positive staining for desmin was observed. Significant amounts of serotonin and its metabolite were detectable. Retinoic acid but not nerve growth factor was able to induce differentiation towards a neuronal phenotype. In summary, TB cells represent primitive neuroectodermal cells having the potential for neuronal, myoblastic and possibly melanoblastic differentiation.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(99)01197-X