The Growth Factor Environment Defines Distinct Pluripotent Ground States in Novel Blastocyst-Derived Stem Cells

Pluripotent stem cell lines can be derived from blastocyst embryos, which yield embryonic stem cell lines (ES cells), as well as the postimplantation epiblast, which gives rise to epiblast stem cell lines (EpiSCs). Remarkably, ES cells and EpiSCs display profound differences in the combination of gr...

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Bibliographic Details
Published in:Cell 2008-10, Vol.135 (3), p.449-461
Main Authors: Chou, Yu-Fen, Chen, Hsu-Hsin, Eijpe, Maureen, Yabuuchi, Akiko, Chenoweth, Joshua G., Tesar, Paul, Lu, Jun, McKay, Ronald D.G., Geijsen, Niels
Format: Article
Language:English
Subjects:
Animals
Blastocyst - cytology
Bone Morphogenetic Protein 4 - metabolism
Cadherins - metabolism
Cell Culture Techniques
Cell Differentiation
Cell Line
Embryo, Mammalian - cytology
Germ Layers - cytology
Intercellular Signaling Peptides and Proteins - metabolism
Mice
Pluripotent Stem Cells - cytology
STEMCELL
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Summary:Pluripotent stem cell lines can be derived from blastocyst embryos, which yield embryonic stem cell lines (ES cells), as well as the postimplantation epiblast, which gives rise to epiblast stem cell lines (EpiSCs). Remarkably, ES cells and EpiSCs display profound differences in the combination of growth factors that maintain their pluripotent state. Molecular and functional differences between these two stem cell types demonstrate that the tissue of origin and/or the growth factor milieu may be important determinants of the stem cell identity. We explored how developmental stage of the tissue of origin and culture growth factor conditions affect the stem cell pluripotent state. Our findings indicate that novel stem cell lines, with unique functional and molecular properties, can be generated from murine blastocyst embryos. We demonstrate that the culture growth factor environment and cell-cell interaction play a critical role in defining several unique and stable stem cell ground states.
ISSN:0092-8674
1097-4172
DOI:10.1016/j.cell.2008.08.035