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Current Clamp and Modeling Studies of Low-Threshold Calcium Spikes in Cells of the Cat's Lateral Geniculate Nucleus
1 Department of Neurobiology, State University of New York, Stony Brook, 11794-5230; and 2 Center for Neural Science and Courant Institute of Mathematical Sciences, New York University, New York, New York 10003 Zhan, X. J., C. L. Cox, J. Rinzel, and S. Murray Sherman. Current Clamp and Modeling...
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| Published in: | Journal of neurophysiology 1999-05, Vol.81 (5), p.2360-2373 |
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| creator | Zhan, X. J Cox, C. L Rinzel, J Sherman, S. Murray |
| description | 1 Department of Neurobiology, State
University of New York, Stony Brook, 11794-5230; and
2 Center for Neural Science and Courant Institute
of Mathematical Sciences, New York University, New York, New York 10003
Zhan, X. J.,
C. L. Cox,
J. Rinzel, and
S.
Murray Sherman.
Current Clamp and Modeling Studies of Low-Threshold Calcium
Spikes in Cells of the Cat's Lateral Geniculate Nucleus. J. Neurophysiol. 81: 2360-2373, 1999. Current clamp and modeling studies of low-threshold calcium
spikes in cells of the cat's lateral geniculate nucleus. All
thalamic relay cells display a voltage-dependent low-threshold
Ca 2+ spike that plays an important role in relay of
information to cortex. We investigated activation properties of this
spike in relay cells of the cat's lateral geniculate nucleus using the combined approach of current-clamp intracellular recording from thalamic slices and simulations with a reduced model based on voltage-clamp data. Our experimental data from 42 relay cells showed
that the actual Ca 2+ spike activates in a nearly
all-or-none manner and in this regard is similar to the conventional
Na + /K + action potential except that its voltage
dependency is more hyperpolarized and its kinetics are slower. When the
cell's membrane potential was hyperpolarized sufficiently to
deinactivate much of the low-threshold Ca 2+ current
( I T ) underlying the Ca 2+ spike,
depolarizing current injections typically produced a purely ohmic
response when subthreshold and a full-blown Ca 2+ spike of
nearly invariant amplitude when suprathreshold. The transition between
the ohmic response and activated Ca 2+ spikes was abrupt and
reflected a difference in depolarizing inputs of |
| doi_str_mv | 10.1152/jn.1999.81.5.2360 |
| format | article |
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University of New York, Stony Brook, 11794-5230; and
2 Center for Neural Science and Courant Institute
of Mathematical Sciences, New York University, New York, New York 10003
Zhan, X. J.,
C. L. Cox,
J. Rinzel, and
S.
Murray Sherman.
Current Clamp and Modeling Studies of Low-Threshold Calcium
Spikes in Cells of the Cat's Lateral Geniculate Nucleus. J. Neurophysiol. 81: 2360-2373, 1999. Current clamp and modeling studies of low-threshold calcium
spikes in cells of the cat's lateral geniculate nucleus. All
thalamic relay cells display a voltage-dependent low-threshold
Ca 2+ spike that plays an important role in relay of
information to cortex. We investigated activation properties of this
spike in relay cells of the cat's lateral geniculate nucleus using the combined approach of current-clamp intracellular recording from thalamic slices and simulations with a reduced model based on voltage-clamp data. Our experimental data from 42 relay cells showed
that the actual Ca 2+ spike activates in a nearly
all-or-none manner and in this regard is similar to the conventional
Na + /K + action potential except that its voltage
dependency is more hyperpolarized and its kinetics are slower. When the
cell's membrane potential was hyperpolarized sufficiently to
deinactivate much of the low-threshold Ca 2+ current
( I T ) underlying the Ca 2+ spike,
depolarizing current injections typically produced a purely ohmic
response when subthreshold and a full-blown Ca 2+ spike of
nearly invariant amplitude when suprathreshold. The transition between
the ohmic response and activated Ca 2+ spikes was abrupt and
reflected a difference in depolarizing inputs of <1 mV. However,
activation of a full-blown Ca 2+ spike was preceded by a
slower period of depolarization that was graded with the amplitude of
current injection, and the full-blown Ca 2+ spike activated
when this slower depolarization reached a sufficient membrane
potential, a quasithreshold. As a result, the latency of the evoked
Ca 2+ spike became less with stronger activating inputs
because a stronger input produced a stronger depolarization that
reached the critical membrane potential earlier. Although
Ca 2+ spikes were activated in a nearly all-or-none manner
from a given holding potential, their actual amplitudes were related to
these holding potentials, which, in turn, determined the level of
I T deinactivation. Our simulations could
reproduce all of the main experimental observations. They further
suggest that the voltage-dependent K + conductance
underlying I A , which is known to delay firing in many cells, does not seem to contribute to the variable latency seen in
activation of Ca 2+ spikes. Instead the simulations indicate
that the activation of I T starts initially with
a slow and graded depolarization until enough of the underling
transient (or T) Ca 2+ channels are recruited to produce a
fast, "autocatalytic" depolarization seen as the Ca 2+
spike. This can produce variable latency dependent on the strength of
the initial activation of T channels. The nearly all-or-none nature of
Ca 2+ spike activation suggests that when a burst of action
potentials normally is evoked as a result of a Ca 2+ spike
and transmitted to cortex, this signal is largely invariant with the
amplitude of the input activating the relay cell.</description><identifier>ISSN: 0022-3077</identifier><identifier>EISSN: 1522-1598</identifier><identifier>DOI: 10.1152/jn.1999.81.5.2360</identifier><identifier>PMID: 10322072</identifier><language>eng</language><publisher>United States: Am Phys Soc</publisher><subject>Action Potentials - physiology ; Animals ; Calcium - physiology ; Cats ; Computer Simulation ; Differential Threshold - physiology ; Female ; Geniculate Bodies - cytology ; Geniculate Bodies - physiology ; Male ; Models, Neurological ; Neurons - physiology ; Patch-Clamp Techniques ; Reaction Time - physiology ; Thalamus - cytology ; Thalamus - physiology</subject><ispartof>Journal of neurophysiology, 1999-05, Vol.81 (5), p.2360-2373</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-a0fd459e5fb6c171af342990e894899cf831072c88a96a49cfbb73eca965bb353</citedby><cites>FETCH-LOGICAL-c470t-a0fd459e5fb6c171af342990e894899cf831072c88a96a49cfbb73eca965bb353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27906,27907</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10322072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhan, X. J</creatorcontrib><creatorcontrib>Cox, C. L</creatorcontrib><creatorcontrib>Rinzel, J</creatorcontrib><creatorcontrib>Sherman, S. Murray</creatorcontrib><title>Current Clamp and Modeling Studies of Low-Threshold Calcium Spikes in Cells of the Cat's Lateral Geniculate Nucleus</title><title>Journal of neurophysiology</title><addtitle>J Neurophysiol</addtitle><description> 1 Department of Neurobiology, State
University of New York, Stony Brook, 11794-5230; and
2 Center for Neural Science and Courant Institute
of Mathematical Sciences, New York University, New York, New York 10003
Zhan, X. J.,
C. L. Cox,
J. Rinzel, and
S.
Murray Sherman.
Current Clamp and Modeling Studies of Low-Threshold Calcium
Spikes in Cells of the Cat's Lateral Geniculate Nucleus. J. Neurophysiol. 81: 2360-2373, 1999. Current clamp and modeling studies of low-threshold calcium
spikes in cells of the cat's lateral geniculate nucleus. All
thalamic relay cells display a voltage-dependent low-threshold
Ca 2+ spike that plays an important role in relay of
information to cortex. We investigated activation properties of this
spike in relay cells of the cat's lateral geniculate nucleus using the combined approach of current-clamp intracellular recording from thalamic slices and simulations with a reduced model based on voltage-clamp data. Our experimental data from 42 relay cells showed
that the actual Ca 2+ spike activates in a nearly
all-or-none manner and in this regard is similar to the conventional
Na + /K + action potential except that its voltage
dependency is more hyperpolarized and its kinetics are slower. When the
cell's membrane potential was hyperpolarized sufficiently to
deinactivate much of the low-threshold Ca 2+ current
( I T ) underlying the Ca 2+ spike,
depolarizing current injections typically produced a purely ohmic
response when subthreshold and a full-blown Ca 2+ spike of
nearly invariant amplitude when suprathreshold. The transition between
the ohmic response and activated Ca 2+ spikes was abrupt and
reflected a difference in depolarizing inputs of <1 mV. However,
activation of a full-blown Ca 2+ spike was preceded by a
slower period of depolarization that was graded with the amplitude of
current injection, and the full-blown Ca 2+ spike activated
when this slower depolarization reached a sufficient membrane
potential, a quasithreshold. As a result, the latency of the evoked
Ca 2+ spike became less with stronger activating inputs
because a stronger input produced a stronger depolarization that
reached the critical membrane potential earlier. Although
Ca 2+ spikes were activated in a nearly all-or-none manner
from a given holding potential, their actual amplitudes were related to
these holding potentials, which, in turn, determined the level of
I T deinactivation. Our simulations could
reproduce all of the main experimental observations. They further
suggest that the voltage-dependent K + conductance
underlying I A , which is known to delay firing in many cells, does not seem to contribute to the variable latency seen in
activation of Ca 2+ spikes. Instead the simulations indicate
that the activation of I T starts initially with
a slow and graded depolarization until enough of the underling
transient (or T) Ca 2+ channels are recruited to produce a
fast, "autocatalytic" depolarization seen as the Ca 2+
spike. This can produce variable latency dependent on the strength of
the initial activation of T channels. The nearly all-or-none nature of
Ca 2+ spike activation suggests that when a burst of action
potentials normally is evoked as a result of a Ca 2+ spike
and transmitted to cortex, this signal is largely invariant with the
amplitude of the input activating the relay cell.</description><subject>Action Potentials - physiology</subject><subject>Animals</subject><subject>Calcium - physiology</subject><subject>Cats</subject><subject>Computer Simulation</subject><subject>Differential Threshold - physiology</subject><subject>Female</subject><subject>Geniculate Bodies - cytology</subject><subject>Geniculate Bodies - physiology</subject><subject>Male</subject><subject>Models, Neurological</subject><subject>Neurons - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Reaction Time - physiology</subject><subject>Thalamus - cytology</subject><subject>Thalamus - physiology</subject><issn>0022-3077</issn><issn>1522-1598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhS0EokvhB3BBPtFTgh3HSXxEUVsqLXDocrYcZ7Lx4sTBjlX23-Nlq8IFcbJn5ntPYz-E3lKSU8qLD4c5p0KIvKE5zwtWkWdok_pFRrlonqMNIenOSF1foFchHAghNSfFS3RBCSsKUhcbFNroPcwrbq2aFqzmHn92PVgz7_H9GnsDAbsBb91Dths9hNHZHrfKahMnfL-Y72luZtyCtb_BdYQ0Xq8C3qoVvLL4Fmajo00V_hK1hRheoxeDsgHePJ6X6NvN9a79lG2_3t61H7eZLmuyZooMfckF8KGrNK2pGlhZCEGgEWUjhB4aRtMbdNMoUakyNbquZqBTxbuOcXaJ3p99F-9-RAirnEzQaVM1g4tBViJ9h6jK_4K0ZoSVVCSQnkHtXQgeBrl4Myl_lJTIUyTyMMtTJLKhkstTJEnz7tE8dhP0fynOGSSAnYHR7McH40Eu4zEYZ93-KG-itTv4uSbjJ0u59ENSXf1blbb4s8Avi5-nqA</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Zhan, X. J</creator><creator>Cox, C. L</creator><creator>Rinzel, J</creator><creator>Sherman, S. Murray</creator><general>Am Phys Soc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19990501</creationdate><title>Current Clamp and Modeling Studies of Low-Threshold Calcium Spikes in Cells of the Cat's Lateral Geniculate Nucleus</title><author>Zhan, X. J ; Cox, C. L ; Rinzel, J ; Sherman, S. Murray</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-a0fd459e5fb6c171af342990e894899cf831072c88a96a49cfbb73eca965bb353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Action Potentials - physiology</topic><topic>Animals</topic><topic>Calcium - physiology</topic><topic>Cats</topic><topic>Computer Simulation</topic><topic>Differential Threshold - physiology</topic><topic>Female</topic><topic>Geniculate Bodies - cytology</topic><topic>Geniculate Bodies - physiology</topic><topic>Male</topic><topic>Models, Neurological</topic><topic>Neurons - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Reaction Time - physiology</topic><topic>Thalamus - cytology</topic><topic>Thalamus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhan, X. J</creatorcontrib><creatorcontrib>Cox, C. L</creatorcontrib><creatorcontrib>Rinzel, J</creatorcontrib><creatorcontrib>Sherman, S. Murray</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurophysiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhan, X. J</au><au>Cox, C. L</au><au>Rinzel, J</au><au>Sherman, S. Murray</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Current Clamp and Modeling Studies of Low-Threshold Calcium Spikes in Cells of the Cat's Lateral Geniculate Nucleus</atitle><jtitle>Journal of neurophysiology</jtitle><addtitle>J Neurophysiol</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>81</volume><issue>5</issue><spage>2360</spage><epage>2373</epage><pages>2360-2373</pages><issn>0022-3077</issn><eissn>1522-1598</eissn><abstract> 1 Department of Neurobiology, State
University of New York, Stony Brook, 11794-5230; and
2 Center for Neural Science and Courant Institute
of Mathematical Sciences, New York University, New York, New York 10003
Zhan, X. J.,
C. L. Cox,
J. Rinzel, and
S.
Murray Sherman.
Current Clamp and Modeling Studies of Low-Threshold Calcium
Spikes in Cells of the Cat's Lateral Geniculate Nucleus. J. Neurophysiol. 81: 2360-2373, 1999. Current clamp and modeling studies of low-threshold calcium
spikes in cells of the cat's lateral geniculate nucleus. All
thalamic relay cells display a voltage-dependent low-threshold
Ca 2+ spike that plays an important role in relay of
information to cortex. We investigated activation properties of this
spike in relay cells of the cat's lateral geniculate nucleus using the combined approach of current-clamp intracellular recording from thalamic slices and simulations with a reduced model based on voltage-clamp data. Our experimental data from 42 relay cells showed
that the actual Ca 2+ spike activates in a nearly
all-or-none manner and in this regard is similar to the conventional
Na + /K + action potential except that its voltage
dependency is more hyperpolarized and its kinetics are slower. When the
cell's membrane potential was hyperpolarized sufficiently to
deinactivate much of the low-threshold Ca 2+ current
( I T ) underlying the Ca 2+ spike,
depolarizing current injections typically produced a purely ohmic
response when subthreshold and a full-blown Ca 2+ spike of
nearly invariant amplitude when suprathreshold. The transition between
the ohmic response and activated Ca 2+ spikes was abrupt and
reflected a difference in depolarizing inputs of <1 mV. However,
activation of a full-blown Ca 2+ spike was preceded by a
slower period of depolarization that was graded with the amplitude of
current injection, and the full-blown Ca 2+ spike activated
when this slower depolarization reached a sufficient membrane
potential, a quasithreshold. As a result, the latency of the evoked
Ca 2+ spike became less with stronger activating inputs
because a stronger input produced a stronger depolarization that
reached the critical membrane potential earlier. Although
Ca 2+ spikes were activated in a nearly all-or-none manner
from a given holding potential, their actual amplitudes were related to
these holding potentials, which, in turn, determined the level of
I T deinactivation. Our simulations could
reproduce all of the main experimental observations. They further
suggest that the voltage-dependent K + conductance
underlying I A , which is known to delay firing in many cells, does not seem to contribute to the variable latency seen in
activation of Ca 2+ spikes. Instead the simulations indicate
that the activation of I T starts initially with
a slow and graded depolarization until enough of the underling
transient (or T) Ca 2+ channels are recruited to produce a
fast, "autocatalytic" depolarization seen as the Ca 2+
spike. This can produce variable latency dependent on the strength of
the initial activation of T channels. The nearly all-or-none nature of
Ca 2+ spike activation suggests that when a burst of action
potentials normally is evoked as a result of a Ca 2+ spike
and transmitted to cortex, this signal is largely invariant with the
amplitude of the input activating the relay cell.</abstract><cop>United States</cop><pub>Am Phys Soc</pub><pmid>10322072</pmid><doi>10.1152/jn.1999.81.5.2360</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of neurophysiology, 1999-05, Vol.81 (5), p.2360-2373 |
| issn | 0022-3077 1522-1598 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69750964 |
| source | American Physiological Society:Jisc Collections:American Physiological Society Journals ‘Read Publish & Join’ Agreement:2023-2024 (Reading list); American Physiological Society Free |
| subjects | Action Potentials - physiology Animals Calcium - physiology Cats Computer Simulation Differential Threshold - physiology Female Geniculate Bodies - cytology Geniculate Bodies - physiology Male Models, Neurological Neurons - physiology Patch-Clamp Techniques Reaction Time - physiology Thalamus - cytology Thalamus - physiology |
| title | Current Clamp and Modeling Studies of Low-Threshold Calcium Spikes in Cells of the Cat's Lateral Geniculate Nucleus |
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