Cloning and characterisation of cDNA sequences encoding for anti-lipopolysaccharide factors (ALFs) in Brazilian palaemonid and penaeid shrimps

Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides found in limulids and crustaceans that have a potent and broad range of antimicrobial activity. We report here the identification and molecular characterisation of new sequences encoding for ALFs in the haemocytes of the freshwater pr...

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Bibliographic Details
Published in:Fish & shellfish immunology 2008-11, Vol.25 (5), p.693-696
Main Authors: Rosa, Rafael Diego, Stoco, Patricia Hermes, Barracco, Margherita Anna
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Anti-lipopolysaccharide factor
Antimicrobial peptide
Brazil
Cloning, Molecular
DNA, Complementary - genetics
Farfantepenaeus paulensis
Freshwater prawn
Gene Expression Regulation - physiology
Invertebrate Hormones - genetics
Invertebrate Hormones - metabolism
Litopenaeus schmitti
Macrobrachium olfersi
Molecular cloning
Molecular Sequence Data
Palaemonidae - metabolism
Penaeid shrimp
Penaeidae - metabolism
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Summary:Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides found in limulids and crustaceans that have a potent and broad range of antimicrobial activity. We report here the identification and molecular characterisation of new sequences encoding for ALFs in the haemocytes of the freshwater prawn Macrobrachium olfersi and also in two Brazilian penaeid species, Farfantepenaeus paulensis and Litopenaeus schmitti. All obtained sequences encoded for highly cationic peptides containing two conserved cysteine residues flanking a putative LPS-binding domain. They exhibited a significant amino acid similarity with crustacean and limulid ALF sequences, especially with those of penaeid shrimps. This is the first identification of ALF in a freshwater prawn.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2008.08.001