Isotachophoretic determination of 3-methylhistidine

A capillary isotachophoretic (cITP) method to determine the concentration of 3-methylhistidine (3-MeHis) in meat and meat products is described. A clear separation of the 3-MeHis from histidine, 1-methylhistidine and other components of acidic sample hydrolyzate was achieved within 20 min. Method ch...

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Bibliographic Details
Published in:Journal of Chromatography A 1999-04, Vol.838 (1), p.191-195
Main Author: Kvasnicka, F
Format: Article
Language:English
Subjects:
Amino acids
Creatinine
Electrophoresis, Capillary - methods
Histidine
Histidine - analysis
Histidine - chemistry
Hydrogen-Ion Concentration
Hydrolysis
Meat - analysis
Meat Products - analysis
Methylhistidines
Methylhistidines - analysis
Methylhistidines - chemistry
Sensitivity and Specificity
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Summary:A capillary isotachophoretic (cITP) method to determine the concentration of 3-methylhistidine (3-MeHis) in meat and meat products is described. A clear separation of the 3-MeHis from histidine, 1-methylhistidine and other components of acidic sample hydrolyzate was achieved within 20 min. Method characteristics (linearity, accuracy, precision and detection limit) were determined. Low laboriousness, sufficient sensitivity and low running cost are the important attributes of cITP method. The developed method was successfully applied to analyses of real samples and used for the determination of lean meat content in meat and meat products.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00122-3