Occurrence of group A rotavirus mixed P genotypes infections in children living in Goiânia-Goiás, Brazil

Group A rotaviruses (RVA) are the main causing agents of acute gastroenteritis worldwide, having a great impact on childhood mortality in developing countries. The objective of this study was to identify RVA-positive fecal samples with mixed P genotypes by hemi-nested reverse transcriptase-polymeras...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2008-11, Vol.27 (11), p.1065-1069
Main Authors: Freitas, E. R. L., Soares, C. M. A., Fiaccadori, F. S., Souza, M., Parente, J. A., Costa, P. S. S., Cardoso, D. D. P.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Brazil
Child
Child mortality
Children
Developing countries
Feces
Feces - virology
Female
Gastroenteritis
Gastroenteritis - virology
Genomes
Genotype
Genotype & phenotype
Genotypes
Group a rotavirus
Humans
Infections
Infectious diseases
Internal Medicine
LDCs
Male
Medical Microbiology
Medical sciences
Mixed infection
Mortality
Phylogeny
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - methods
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus - isolation & purification
Rotavirus Infections - virology
Sequence Analysis, DNA
Sequence Homology
Viral diseases
Viruses
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Summary:Group A rotaviruses (RVA) are the main causing agents of acute gastroenteritis worldwide, having a great impact on childhood mortality in developing countries. The objective of this study was to identify RVA-positive fecal samples with mixed P genotypes by hemi-nested reverse transcriptase-polymerase chain reaction (RT-PCR), followed by sequencing confirmation. Our results showed that, from the 81 RVA-positive samples, 25 were positive for more than one P genotype by hemi-nested RT-PCR. Of these 25 samples, 12 (48%) had their mixed P genotypes confirmed by sequencing and, from these, 10 were identified as P[6]P[8], one as P[4]P[6], and one as P[4]P[6]P[8]. Our results confirm the occurrence of RVA mixed infections among children in Brazil and reinforce the importance of the constant monitoring of RVA circulating strains for the efficacy of control/prevention against these agents.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-008-0542-2